Bioremediation and Detoxification of Xenobiotic Organic Compounds in Landfill Leachate by Pseudomonas sp. ISTDF1

  • Pooja Ghosh
  • Mayank Krishna
  • Mihir Tanay Das
  • Indu Shekhar Thakur


Increase in municipal solid waste (MSW) production has been a consequence of rapid population growth and urbanization in the past decade. It is startling to know that presently Delhi itself generates 8000 tonnes/day of MSW and to make the situation worse, it is projected to rise to 17,000-25,000 tonnes/day by the year 2021 (Talyan et al., 2008). The most commonly employed method for MSW management is landfill disposal. Landfill requires a close environmental engineering surveillance in its design and operation, as it is likely to generate leachate which would potentially contaminate nearby ground water and surface water (Mor et al., 2006). In spite of being economically viable, generation of heavily polluted leachate constitutes a major drawback of landfills. In the absence of leachate and landfill gas collection systems, these landfills are a major source of groundwater contamination and air pollution, including the generation of greenhouse gases (Talyan et al., 2008; Gardner et al., 1993).


Municipal Solid Waste Comet Assay Phthalic Acid Landfill Leachate Tail Moment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by research grant from Council of Scientific and Industrial Research (CSIR), New Delhi, India. We thank Mr. Ajai Kumar of Advanced Instrumentation Research Facility (AIRF), JNU, New Delhi for GC-MS analysis and Century Pulp and Paper Mill, Lalkuan, Uttarakhand, for providing effluent and sludge/sediments.


  1. Chang, E.E., Miao, Z.F., Lee, W.J., Chao, H.R., Li, L.A., Wang, Y.F., Ko, Y.C., Tsai, F.Y., Yeh, S.C. and Tsou, T.C. (2007). Arecoline inhibits the 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced cytochrome P450 1A1 activation in human hepatoma cells. Journal of Hazardous Materials, 146: 356-361.CrossRefGoogle Scholar
  2. Cozzarelli, I.M., Böhlke, J.K., Masoner, J., Breit, G.N., Lorah, M.M., Tuttle, M.L.W. and Jaeschke, J.B. (2011). Biogeochemical evolution of a landfill leachate plume. Ground Water, 49: 663-687.CrossRefGoogle Scholar
  3. Gardner, N., Manley, B.J.W. and Pearson, J.M. (1993). Gas emissions from landfills and their contributions to global warming. Energy and Environmental Management, 44: 165-174.Google Scholar
  4. Hu, J., Nakamura, J., Richardson S.D. and Aitken M.D. (2012). Evaluating the effects of bioremediation on genotoxicity of polycyclic aromatic hydrocarbon-contaminated soil using genetically engineered, higher eukaryotic cell lines. Environmental Science and Technology, 46: 4607-4613.CrossRefGoogle Scholar
  5. Huset, C.A., Barlaz, M.A., Barofsky, D.F. and Field, J.A. (2011). Quantitative determination of fluorochemicals in municipal landfill leachates. Chemosphere, 82: 1380-1386.CrossRefGoogle Scholar
  6. Iida, T., Mukouzaka, Y., Nakamura, K. and Kudo, T. (2002). Plasmid borne genes code for an angular dioxygenase involved in dibenzofuran degradation by Terrabacter sp. strain YK3. Applied Environmental Microbiology, 68: 3716-3723.CrossRefGoogle Scholar
  7. Jaiswal, P.K., Kohli, S., Gopal, M. and Thakur, I.S. (2011). Isolation and characterization of alkalotolerant Pseudomonas sp. strain ISTDF1 for degradation of dibenzofuran. Journal of Industrial Microbiology and Biotechnology, 38: 503-511.Google Scholar
  8. Kapoor, A. and Viraraghavan, T. (1995). Fungal Biosorption – An alternative treatment option for heavy metal bearing wastewaters: A review. Bioresource Technology, 53: 195-206.Google Scholar
  9. Kim, T.J., Lee, E.Y., Kim, Y.J., Cho, K.S. and Ryu, H.W. (2003). Degradation of polycyclic aromatic hydrocarbons by Burkholderis cepacia 2A-12. World Journal of Microbial Biotechnology, 19: 411-417.CrossRefGoogle Scholar
  10. Mor, S., Ravindra, K., Dahiya, R.P. and Chandra, A. (2006). Leachate Characterization and Assessment of Groundwater Pollution near Municipal Solid Waste Landfill Site. Environmental Monitoring and Assessment, 118: 435-456.CrossRefGoogle Scholar
  11. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65: 55-63.CrossRefGoogle Scholar
  12. Nwagbara, O., Darling-Reed, S.F., Tucker, A., Harris, C., Abazinge, M., Thomas, R.D. and Gragg, R.D. (2007). Induction of cell death, DNA strand breaks, and cell cycle arrest in DU145 human prostate carcinoma cell line by benzo[a]pyrene and benzo[a]pyrene-7,8-diol-9,10-epoxide. International Journal of Environmental Research and Public Health, 4: 10-14.CrossRefGoogle Scholar
  13. Resnick, S.M. and Gibson, D.T. (1996). Regio- and steriospecific oxidation of fluorene, dibenzofuran and dibenzothiophene by naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. Applied Environmental Microbiology, 62: 4073-4080.Google Scholar
  14. Seo, J.S., Keum, Y.S. and Li, Q.X. (2009). Bacterial degradation of aromatic compounds. International Journal of Environmental Research and Public Health, 6: 278-309.CrossRefGoogle Scholar
  15. Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175: 184-191.CrossRefGoogle Scholar
  16. Slack, R.J. (2005). Household hazardous waste in municipal landfills: Contaminants in leachate. Science of the Total Environment, 337: 119-137.CrossRefGoogle Scholar
  17. Tai, H.L., Dehn, P.F. and Olson, J.R. (1994). Use of rat H4IIE and human HepG2 cells as models to investigate the metabolism of 2, 3, 7, 8-tetrachlorodibenzofuran (TCDF). Toxicologist, 14: 271-278.Google Scholar
  18. Talyan, V., Dahiya, R.P. and Sreekrishnan, T.R. (2008). State of municipal solid waste management in Delhi, the capital of India. Waste Management, 28: 1276-1287.CrossRefGoogle Scholar
  19. Widziewicz, K., Kalka, J., Skonieczna, M. and Madej, P. (2012). The Comet Assay for the Evaluation of Genotoxic Potential of Landfill Leachate. The Scientific World Journal, doi: 10.1100/2012/435239.Google Scholar

Copyright information

© Capital Publishing Company 2015

Authors and Affiliations

  • Pooja Ghosh
    • 1
  • Mayank Krishna
    • 1
  • Mihir Tanay Das
    • 1
  • Indu Shekhar Thakur
    • 1
  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

Personalised recommendations