Abstract
Heterochromatin is a conserved chromatin arrangement with roles in transcriptional regulation and genome integrity. It is characterized by regular nucleosomal arrays decorated with specific histone post-translational modifications. These recruit homologs of the HP1 protein that mediate the formation of a tightly compacted chromatin structure refractory to virtually all nucleic acid transactions. Heterochromatin fibers organize into higher order structures that shape a large part of subnuclear organization. Heterochromatin coats a diverse set of genetic elements whose only common feature is their repetitive nature. These include transposable elements (TE), satellite repeats, and subtelomeric repeats. The mechanisms that target these elements for compaction into heterochromatin have been elusive for a long time, with tantalizing indications of a central role for RNA in this process. In the last decade, work in model organisms from fungi to plants and metazoans have revealed the highly dynamic nature of heterochromatin, and unexpected relationships with fundamental cellular processes, guided by non-coding RNA. The deepest mechanistic insights into this phenomenon have come from the study of heterochromatin in the fission yeast Schizosaccharomyces pombe. In particular, the heterochromatin that covers pericentric repeats has been intensely scrutinized, because of the tandem repeat structure reminiscent of centromeres of higher organisms. This chapter summarizes the current view of the organization and regulation of pericentric heterochromatin in S. pombe with a focus on involvement of non-coding RNA.
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Zaratiegui, M. (2014). Regulation of Pericentric Heterochromatin by ncRNA in Schizosaccharomyces pombe . In: Sesma, A., von der Haar, T. (eds) Fungal RNA Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-05687-6_13
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