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Capillary Electrophoresis Microchip for DNA Amperometric Detection Featuring Agarose Gel

  • M. H. Ghanim
  • M. Z. Abdullah
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 42)

Abstract

One of the important factors influencing the separation efficiency and sensitivity of the capillary electrophoresis microchip is the background material. In this work a microchip capillary electrophoresis (MCE) featuring Agarose gel as a separating medium was fabricated and tested in the laboratory. The design was based on polydimethysiloxane (PDMS) engraved microchannel with platinum electrodes etched onto glass substrate. Operated under relatively low electric field of 24 V/cm and sample volume of 1.5 μl, the new device was able to separate DNA fragments with resolution consistently higher than 100 nA. Separation is achieved in less than 45 minutes.

Keywords

Microchip Capillary Electrophoresis amperemetric detector DNA analysis 

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • M. H. Ghanim
    • 1
  • M. Z. Abdullah
    • 1
  1. 1.School of Electrical and Electronic EngineeringUniversiti Sains MalaysiaPenangMalaysia

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