Identification of Differently Expresed Proteins Related to Drillings Fluids Exposure in Hydractinia Symbiolongicarpus by Mass Spectrometry
Due to the interest of increasing the national oil production in Colombia, seawater exploration it is a real option. During well production, drilling fluids are used to support drilling boreholes into the earth or sea platform. Water-based drilling fluid (WBF) can be composed of several materials as clay, barite, emulsifier additives and metal ions. Although there are studies that evaluate mid and long-term effects to exposure to this fluids there is no way to monitor any potential effect on seawater organisms. Hydractinia symbiolongicarpus was used to evaluate the effect of WBF exposure in order to identify possible biomarkers that may aid to monitor seawater organism’s health and prevent any ecosystem disruption. Hydractinia was exposed to WBF and 30 Up-Regulated spot proteins were determined by 2D-DIGE. All spots proteins were identified by mass spectrometry using MASCOT and X! Tandem search engine. Glutathione-S-transferase and peroxiredoxin VI were recognized and proposed as possible biomarker since they are related to cell detoxification and redox homeostasis, respectively.
KeywordsMass spectrometry Hydractinia Drilling fluids biomarkers
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