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Pathways and Signaling Crosstalk with Oxidant in Calcium Influx in Airway Smooth Muscle Cells

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Calcium Signaling In Airway Smooth Muscle Cells

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Abstract

Influx of extracellular calcium through calcium channels in the plasma membrane contributes to the sustained phase of [Ca2+]i elevation following agonist stimulation. The interactions between channel proteins like TRPC, STIM1, and Orai1, as well as protein and regulatory molecules including reactive oxygen species (ROSs), have been shown to be involved in the opening of calcium entry channels. The contribution of endogenous hydrogen peroxide (H2O2) to agonist-induced intracellular calcium oscillation was first reported as early as 2002 in vascular endothelial cells. While both the receptor-operated calcium channel and store-operated calcium channel proteins possess cysteine residues, they could also be potential targets of ROSs. Mitochondria-derived H2O2 was shown to work as a cofactor together with STIM1 to mediate histamine-stimulated Ca2+ influx in airway smooth muscle cells. Furthermore, intracellular ROSs can interact with members of transient receptor potential (TRP) superfamilies and contribute to Ca2+ influx. ROS-induced TRP intracellular translocation has been suggested as a critical underlying mechanism of TRP activation, which could be a clue to explain how intracellular ROSs cooperate with STIM1 to mediate Ca2+ influx. The cooperation of ROSs and calcium signaling has magnified physiological or pathological effects.

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Abbreviations

[Ca2+]i :

intracellular calcium concentration

ASMC:

airway smooth muscle cell

SR:

sarcoplasmic reticulum

IP3 :

inositol 1,4,5-trisphosphate

ROS:

reactive oxygen species

VDCC:

voltage-dependent calcium channels

ROC:

receptor-operated calcium channel

SOC:

store-operated calcium channel

CCE:

capacitative Ca2+ entry

PIP2 :

phosphatidylinositol biphosphate

Tg:

thapsigragin

ICRAC :

calcium-release-activated calcium current

TRP:

transient receptor potential

STIM:

stromal interaction molecule

TRPC:

TRP canonical

Trx:

thioredoxin

CRAC:

Ca2+ release-activated channel

SCID:

severe combined immune deficiency

NADPH:

nicotinamide adenine dinucleotide phosphate

RNS:

reactive nitrogen species

RyR:

ryanodine receptor

mtDNA:

mitochondrial DNA

EB:

ethidium bromide

IGF-1:

insulin-like growth factor 1

EGF:

endothelial growth factor

AVP:

arginine vasopressin

VCAM:

vascular cell adhesion molecule

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Authors and Affiliations

  1. Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People’s Republic of China

    Lei Cai & Qinghua Hu

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  1. Lei Cai
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  2. Qinghua Hu
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Correspondence to Qinghua Hu .

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  1. Albany Medical College Center for Cardiovascular Sciences, Albany, New York, USA

    Yong-Xiao Wang

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Cai, L., Hu, Q. (2014). Pathways and Signaling Crosstalk with Oxidant in Calcium Influx in Airway Smooth Muscle Cells. In: Wang, YX. (eds) Calcium Signaling In Airway Smooth Muscle Cells. Springer, Cham. https://doi.org/10.1007/978-3-319-01312-1_14

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