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Proteomics in the Systems-Level Study of the Metabolic Syndrome

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Book cover A Systems Biology Approach to Study Metabolic Syndrome

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Abstract

Proteomics has come a long way from the initial qualitative analysis of proteins present in a given sample to the large-scale characterization of proteomes, their interactions and dynamic behaviour in time and space. Originally enabled by breakthroughs in protein separation and visualization (by two-dimensional gels electrophoresis) and protein identification (by mass spectrometry), the discipline now encompasses a large body of protein and peptide separation, labelling, detection and sequencing tools supported by stable-isotope- and label-free techniques and computational data processing for quantitative proteomics. The key functional importance to investigate the protein complement has driven the study of proteomes in numerous physiological and pathological conditions. Proteomics has been mainly applied in discovering novel biomarkers of disease, evidenced by the fact that most clinical tests today measure proteins in blood. Moreover, understanding the proteome helps investigate health and disease states and understand the mechanisms of action of specific molecules, e.g., nutrients or drugs. Here, we briefly recapitulate proteomic technologies and cover their evolution to today’s and future cutting-edge platforms. Then we review and discuss proteomic applications to the study of the metabolic syndrome.

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Authors and Affiliations

  1. BioAnalytical Science Department, Nestlé Research Centre, Vers-chez-les-Blanc, 1000, Lausanne, Switzerland

    Isabel Rubio-Aliaga, Irma Silva-Zolezzi, Michael Affolter & Alexandre Panchaud

  2. Molecular Biomarkers Core, Nestlé Institute of Health Sciences SA, EPFL Campus Quartier de l’innovation, bâtiment H, 1015, Lausanne, Switzerland

    Loïc Dayon & Prof. Martin Kussmann PhD

  3. Faculty of Life Sciences, Ecole Polytechnique Fédérale Lausanne (EPFL), Lausanne, Switzerland

    Prof. Martin Kussmann PhD

  4. Faculty of Science, Aarhus University, Aarhus, Denmark

    Prof. Martin Kussmann PhD

Authors
  1. Isabel Rubio-Aliaga
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  2. Irma Silva-Zolezzi
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  3. Michael Affolter
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  4. Loïc Dayon
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  5. Alexandre Panchaud
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  6. Prof. Martin Kussmann PhD
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Correspondence to Martin Kussmann PhD .

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  1. VTT Technical Research Centre of Finland, Espoo, Finland

    Matej Orešič

  2. Institute of Metabolic Science, University of Cambridge, Cambridge, United Kingdom

    Antonio Vidal-Puig

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Rubio-Aliaga, I., Silva-Zolezzi, I., Affolter, M., Dayon, L., Panchaud, A., Kussmann, M. (2014). Proteomics in the Systems-Level Study of the Metabolic Syndrome. In: Orešič, M., Vidal-Puig, A. (eds) A Systems Biology Approach to Study Metabolic Syndrome. Springer, Cham. https://doi.org/10.1007/978-3-319-01008-3_10

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