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Utilising Ultrafast Lasers for Multiphoton Biomedical Imaging

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Abstract

This chapter covers the benefits and applications of ultrafast laser scanning microscopes from a biomedical perspective. The basic architecture of a laser microscope is discussed, including how to design a laser scanning system with lateral and axial control. Also investigated is the design of custom collection optics for optimizing the detection of emitted photons and maximizing that emitted fluorescence in the presence of photobleaching. In addition, this chapter addresses three techniques novel to the biomedical community. The first is the technique of temporal focusing and its application toward wide-field imaging and micromachining. Also investigated is the concept of photon counting in multiphoton microscopy and how this approach to imaging has become practical for everyday use. Finally, several different methods are revealed for implementing spectral imaging with a multiphoton microscope platform.

Keywords

Photon Counting Multiphoton Microscopy Pulse Amplifier Photon Counting System Laser Clock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This work was funded by the National Institute of Biomedical Imaging and Bioengineering, Grant EB-003832.

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© Springer International Publishing Switzerland 2013

Authors and Affiliations

  1. 1.Center for Microintegrated Optics for Advanced Bioimaging and Control, Department of PhysicsColorado School of MinesGoldenUSA

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