Abstract
The analysis of the expression of various glycosyltransferases by Northern blot analysis and specific enzyme assays has shown their differential tissue expression. On the basis of this the view is held that specific glycan structures in general reflect the expression of the respective glycosyltransferases. Cytochemical in situ studies provide distinct advantages over Northern blot analyses and specific enzyme assays since particular glycans can be localised to specific cell types in organs with complex cellular composition. With the application of lectins and monoclonal antibodies, histochemistry therefore represents an important tool in studies of cell type-specific glycosylation and may provide a clue about their possible functions.
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Pavelka, M., Roth, J. (2010). Glycocalyx: Cell Type Specificity and Domains. In: Functional Ultrastructure. Springer, Vienna. https://doi.org/10.1007/978-3-211-99390-3_85
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DOI: https://doi.org/10.1007/978-3-211-99390-3_85
Publisher Name: Springer, Vienna
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