Abstract
Various cell types including stem cells, epithelial, neuronal and mesenchymal cells as well as different tumour cell types can be grown in vitro in tissue culture dishes or bottles. Routinely, plastic tissue culture dishes are used and cells can survive and multiply when supplied with appropriate culture medium, temperature and atmosphere. Epithelial cells in tissues are polarised and monolayers of polarised epithelial cells can be obtained when grown on porous membranes. Cell cultures provide excellent experimental tools since they can be studied microscopically or analysed biochemically and can be used as host to synthesise and secrete foreign proteins such as monoclonal antibodies and recombinant proteins.
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References
Balcarova-Ständer J, Pfeiffer S, Fuller S, and Simons K (1984) Development of cell surface polarity in the epithelial Madin-Darby canine kidney (MDCK) cell line. EMBO J 3: 2687
Bershadsky A and Vasiliev J (1988) Cytoskeleton. New York: Plenum Press
Bray D (2001) Cell movements: From molecules to motility, 2nd edn. New York: Garland Publishing
Cereijido M, Robbins E, Dolan W, Rotunna C, and Sabatini D (1978) Polarized monolayers formed by epithelial cells on a permeable and translucent support. J Cell Biol 77: 853
Condeelis J (1993) Life at the leading edge: the formation of cell protrusions. Annu Rev Cell Biol 9: 411
Pantaloni D, Le Clainche C, and Carlier M (2001) Mechanism of actin-based motility. Science 292: 1502
Rodriguez-Boulan E and Powell SK (1992) Polarity of epithelial and neuronal cells. Annu Rev Cell Biol 8: 395
Simons K and Fuller S (1985). Cell surface polarity in epithelia. Annu Rev Cell Biol 1: 243
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Pavelka, M., Roth, J. (2010). Cells in Culture. In: Functional Ultrastructure. Springer, Vienna. https://doi.org/10.1007/978-3-211-99390-3_82
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DOI: https://doi.org/10.1007/978-3-211-99390-3_82
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-99389-7
Online ISBN: 978-3-211-99390-3
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