Abstract
The enormous complexity of the Golgi apparatus is easily overlooked when electron microscopic studies are restricted to analyses of single thin sections. Additional studies of semithin and thick sections, analyses of section series, use of eucentric goniometer equipment for controlled tilting of the specimens within the electron microscope, and the introduction of electron tomography into the field of cell biology has tremendously improved the knowledge of the morpho-functional architecture of the Golgi apparatus and its dynamic organisation.
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Baumeister W, Grimm R, and Walz J (1999) Electron tomography of molecules and cells. Trends Cell Biol 9, 81
D’Angelo G, Vicinanza M, and de Matteis MA (2008) Lipid transfer proteins in biosynthetic pathways. Curr Opin Cell Biol 20: 360
Hanada K, Kumagai K, Tomishige N, and Yamaji T (2009) CERT-mediated trafficking of ceramide. Biochim Biophys Acta 1791: 684
Hermo L and Smith CE (1998) The structure of the Golgi apparatus: a sperm’s eye view in principal epithelial cells of the rat epididymis. Histochem Cell Biol 109, 431
Ladinsky MS, Mastronarde DN, McIntosh JR, Howell KE, and Staehelin LA (1999) Golgi structure in three dimensions: functional insights from the normal rat kidney cell. J Cell Biol. 144: 1135
Marsh M (2005) Lessons from tomographic studies of the mammalian Golgi. Biochem Biophys Acta 1744: 273
Munro S (2003) Earthworms and lipid couriers. Nature 426: 775
Polishchuk RS and Mironov AA (2004) Structural aspects of Golgi function. Cell Mol Life Sci 61: 146
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Pavelka, M., Roth, J. (2010). Golgi Apparatus, TGN and Trans Golgi-ER: Tilt Series. In: Functional Ultrastructure. Springer, Vienna. https://doi.org/10.1007/978-3-211-99390-3_37
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DOI: https://doi.org/10.1007/978-3-211-99390-3_37
Publisher Name: Springer, Vienna
Print ISBN: 978-3-211-99389-7
Online ISBN: 978-3-211-99390-3
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