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Hybridisation histochemistry

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Regulatory Peptides

Part of the book series: Experientia Supplementum ((EXS,volume 56))

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Summary

The location of gene expression by hybridisation histochemistry is being applied in many areas of research and diagnosis. The aim of this technique is to detect specific mRNA in cells and tissues by hybridisation with a complementary DNA or RNA probe. Requirements for optimal specificity, sensitivity, resolution and speed of detection may not all be encompassed in one simple technique suitable for all applications, thus appropriate procedures should be selected for specific objectives. With reference to published procedures and our own extensive experience, we have evaluated fixatives, probes, labels and other aspects of the technique critical to the preservation and hybridisation in situ of mRNA and detection and quantitation of hybrids.

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Authors
  1. J. D. Penschow
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  2. J. Haralambidis
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  3. P. E. Darling
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  4. I. A. Darby
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  5. E. M. Wintour
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  6. G. W. Tregear
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  7. J. P. Coghlan
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Editors and Affiliations

  1. Royal Postgraduate Medical School Department of Histochemistry, Hammersmith Hospital, Du Cane Road, London, W12 0HS, England

    Julia M. Polak

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© 1989 Birkhäuser Verlag

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Penschow, J.D. et al. (1989). Hybridisation histochemistry. In: Polak, J.M. (eds) Regulatory Peptides. Experientia Supplementum, vol 56. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9136-3_4

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  • DOI: https://doi.org/10.1007/978-3-0348-9136-3_4

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-7643-1976-2

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