Summary
BSA incubated in solution in presence of several superoxide producing systems acquired peroxide groups which were detected by an iodometric assay. Activated neutrophils, xanthine oxidase acting on xanthine in presence of Fe (III), NADH oxidase with NADH and Fe (III) or Cu (II), and the combination of ascorbate/Fe(II)/EDTA were all effective. The role of Fenton reaction in the oxidation was shown by its inhibition with SOD, catalase, or absence of metal. Apoprotein B isolated from human LDL oxidised by gamma radiation or by incubation with Cu (II) was also peroxidised. The extent of peroxidation was linear with time, showing that the process was not affected by the normal antioxidants present in the LDL particles.
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References
Amici, A., Levine, R.L., Tsai, L. and Stadtman, E.R. (1989) J. Biol. Chem.264, 3341–3346.
Babiy, A.V., Gebicki, J.M. and Sullivan, D.R. (1990) Atherosclerosis81, 175–182.
Cardin, A.D., Witt, K.R., Barnhart, C.L. and Jackson, R.L. (1982) Biochemistry21, 4503–4511.
Davies, K.J.A., Delsignore, M.E. and Lin, S.W. (1987) J. Bil. Chem.262, 9902–9907.
Esterbauer, H., Dieber-Rotheneder, M., Striegl, G. and Waeg, G. (1991) Am. J. Clin. Nutr.53, 314S–321S.
Esterbauer, H., Dieber-Rotheneder, M., Waeg, G., Striegl, G. and Jurgens, G. (1990) Chem. Res. Toxicol.3, 77–92.
Frei, B., England, L. and Ames, B.N. (1989) Proc. Natl. Acad. Sci. USA86, 6377–6381.
Gebicki, J. M., Jurgens, G. and Esterbauer, H. (1991) in Oxidative Stress: Oxidants and Antioxidants (Sies, H., ed.) pp 371–397, Academic Press.
Halliwell, B. (1988) Biochem. Pharmacol.37, 569–571.
Jessup, W., Rankin, S. M., DeWhalley, C.V., Hoult, J.R.S., Scott, J. and Leake, D.S. (1990) Biochem. J.265, 399–405.
Levine, R.L., Oliver, C.N., Fulks, R.M. and Stadtman, E.R. (1981) Proc. Natl. Acad. Sci. USA,78, 2120–2124.
Lissi, E.A. and Clavero, N. (1990) Free Rad. Res. Comms.10, 177–184.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) J. Biol. Chem.193, 265–275.
O’Donnell, J.H. and Sangster, D.F. (1970) Principles of Radiation Chemistry, Arnold, London.
Okada, S., Kraunz, R. and Gassner, E. (1960) Radiat. Res.13, 607–612.
Oliver, C.N., Levine, R.L. and Stadtman, E.R. (1987a) J. Amer. Geriatric Sci.35, 947–956.
Oliver, C.N., Ahn, B.W., Moerman, E.J., Goldstein, S. and Stadtman, E.R. (1987b) J. Biol. Chem.262, 5488–5491.
Pacifici, R.E. and Davies, K.J.A. (1990) in Methods in Enzymology (Packer, L. and Glazer, A.N., eds.) Vol. 186, pp 485–502, Academic Press, San Diego.
Schuessler, H. and Herget, A. (1980) Int. J. Radiat. Biol.37, 71–80.
Schuessler, H. and Schilling, K. (1984) Int. J. Radiat. Biol.45, 267–281.
Socorro, L. and Camejo, G. (1979) J. Lipid Res.20, 631–638.
Shireman, R., Kilgore, L.L. and Fisher, W.R. (1977) Proc. Natl. Acad. Sci. USA74, 5150–5154.
Simpson, J., Narita, S., Gieseg, S., Gebicki, S., Gebicki, J.M. and Dean, R.T. (1992) Biochem. J.282, 621–624.
Stadtman, E.R. (1986) Trends Biochem. Sci.11, 11–12.
Wayner, D.D.M., Burton, G.W., Ingold, K.U., Barclay, L.R.C. and Locke, S.J. (1987) Biochim. Biophys. Acta924, 408–419.
Winn, J.S., Guille, J., Gebicki, J.M. and Day, R.O. (1991) Biochem. Pharmacol.41, 31–36.
Wolff, S.P. and Dean, R.T. (1986) Biochem. J.234, 399–403.
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© 1993 Birkhäuser Verlag Basel/Switzerland
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Babiy, A.V., Gebicki, S., Gebicki, J.M. (1993). Protein Peroxides: Formation by Superoxide-Generating Systems and During Oxidation of Low Density Lipoprotein. In: Poli, G., Albano, E., Dianzani, M.U. (eds) Free Radicals: from Basic Science to Medicine. Molecular and Cell Biology Updates. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9116-5_29
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DOI: https://doi.org/10.1007/978-3-0348-9116-5_29
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