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Analysis of Free Radicals in Biological Systems pp 261–275Cite as

Methodological and practical aspects for in vitro studies of oxidative stress on cell culture models (toxicity and protection)

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Summary

Cell culture systems are especially useful to study the cytotoxicity mediated by reactive oxygen species (ROS) under defined conditions, and to understand cytoprotection and cellular adaptative response. This article reviews the methodological and practical aspects for studying cell injury using chromium-release assay, loss of cellular “adhesion/proliferation” ability and inactivation of mitochondria1 enzymes in different model systems subjected to intracellular or extracellular oxidative stress. Their advantages, limitations as well as their biological significance are discussed.

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Author information

Authors and Affiliations

  1. Laboratoire de Biochimie des Micronutriments et Radicaux Libres, GREPO, CHRU, F-38043, Grenoble Cédex 3, France

    M. J. Richard & P. Guiraud

Authors
  1. M. J. Richard
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  2. P. Guiraud
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Editor information

Editors and Affiliations

  1. Laboratoire de Biochimie C, CHU Albert Michallon, BP 217, F-38043, Grenoble Cédex 9, France

    A. E. Favier

  2. CEA/Département de Recherche Fondamentale sur la Matière, Condensée, SESAM/LAN, 17, rue des Martyrs, F-38054, Grenoble Cédex 9, France

    J. Cadet

  3. Biophysics Research Institute, Medical College of Wisconsin, 8701 Watertown Plank Road, PO Box 26509, Milwaukee, WI, 53226-0509, USA

    B. Kalyanaraman

  4. Laboratoire de Biochimie Recherche, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    M. Fontecave

  5. Laboratoire de Chimie Biomimétique LEDSS II, Domaine Universitaire, F-38400, Saint Martin d’Hères, France

    J.-L. Pierre

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© 1995 Birkhäuser Verlag Basel/Switzerland

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Richard, M.J., Guiraud, P. (1995). Methodological and practical aspects for in vitro studies of oxidative stress on cell culture models (toxicity and protection). In: Favier, A.E., Cadet, J., Kalyanaraman, B., Fontecave, M., Pierre, JL. (eds) Analysis of Free Radicals in Biological Systems. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9074-8_19

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  • DOI: https://doi.org/10.1007/978-3-0348-9074-8_19

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-9895-9

  • Online ISBN: 978-3-0348-9074-8

  • eBook Packages: Springer Book Archive

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