Summary
In this review a universal thermistor-based biosensor system is described with examples from clinical chemistry, bioprocess monitoring and environmental control. The technique is based on the measurement of the small temperature changes associated with enzymatic reactions occurring in a microreactor with immobilized enzyme. The system has good operational stability and a sensitivity that permits measurements down to 1 µM concentrations. Current developments include devices constructed by micromachining for multisensing purposes and miniaturised instrumentation intended for use in portable monitoring. With use of special supports for enzyme immobilisation even untreated whole blood samples can be applied. Another current line of investigation involves hybrid biosensors, such as combinations of electrochemistry and calorimetry into bioelectrocalorimetric devices with interesting new properties.
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© 1997 Birkhäuser Verlag Basel/Switzerland
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Danielsson, B., Xie, B. (1997). Thermistor-based biosensing. In: Scheller, F.W., Schubert, F., Fedrowitz, J. (eds) Frontiers in Biosensorics II. EXS, vol 81. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-9045-8_5
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DOI: https://doi.org/10.1007/978-3-0348-9045-8_5
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