Abstract
Lung cancer is the result of chronic exposure of a range of normal respiratory epithelial cells to carcinogens. This exposure produces a complicated biology which is reflected by the numerous histologies of lung cancer [1], Currently, the precise sequence of genetic events leading to an invasive cancer is speculative, but the development of new techniques offers possibilities of understanding this process. In addition, these techniques could be instrumental in identifying early markers for the detection of lung tumors before they metastasize [2].
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References
Linnoila RI, Aisner SC (1995) Pathology of lung cancer: an exercise in classification. In: Johnson BE, Johnson DH (eds): Lung cancer, Wiley-Liss, New York, pp 73–95.
Mulshine JL, Scott F, Zhou J, Avis I, Vos M, Miller MJ, Szabo E, Martinez A, Treston AM, Cuttitta F, et al. (1996) Development of molecular approaches to early lung cancer detection. Seminars in Radiation Oncology 6: 12–15.
Saldise I, Martinez A, Montuenga LM, Treston A, Springall RD, Polak JM, Vásquez JJ (1996) Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors. J Histochem Cytochem 44: 3–12.
Martinez A, Treston AM, Saldise L, Montuenga LM, Linnoila RI (1996) Expression of peptidyl-glycine a-amidating mono-oxygenase (PAM) enzymes in morphological abnormalities adjacent to pulmonary tumors. Am J Pathol 149: 707–716.
Martinez A, Miller MJ, Unsworth EJ, Siegfried JM, Cuttitta F (1995) Expression of adrenomedullin in normal human lung and in pulmonary tumors. Endocrinology 136: 4099–4105.
Avis I, Jett M, Boyle T, Vos MD, Moody T, Treston AM, Martinez A, Mulshine JL (1996) Growth control of lung cancer by interruption of 5-lipoxygenase-mediated growth factor signaling. J Clin Invest 97: 806–813.
Miller MJ, Martinez A, Unsworth EJ, Thiele CJ, Moody TW, Elsasser T, Cuttitta F (1996) Adrenomedullin expression in human tumor cell lines, its potential role as an autocrine growth factor. J Biol Chem 271: 23345–23351.
Ebina M, Martinez A, Birrer MJ, Linnoila RI (1995) Topographic mapping of p53 mutations in non-small cell lung cancer, novel approach to carcinogenesis using in situ PCR. Proc Am Assoc Cancer Res 36: 578.
John HA, Birnstiel ML, Jones KW (1969) RN-DNA hybrids at the cytological level. Nature 223: 582–587.
Polak JM, McGee JO’D (eds) (1990): In situ hybridization, principles and practice. Oxford University Press, New York.
Terenghi G, Fallon RA (1990) Techniques and applications of in situ hybridization. In: Underwood JCE (ed) Current topics in pathology. 82: Pathology of the nucleus. Berlin, Springer-Verlag, 289–337.
Speel EJM, Ramaekers FCS, Hopman AHN (1995) Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry: “Who is still afraid of red, green and blue?”. Histochem J 27: 833–858.
Nuovo GJ (ed) (1992) PCR in situ hybridization; protocols and applications. New York: Raven Press.
Corcoran M, Levin M, Jacobson S, Liotta L (1994) From hot starts and false starts to smart starts: in situ PCR. NIH Catalyst (May): 12-14.
Martinez A, Miller MJ, Quinn K, Unsworth EJ, Ebina M, Cuttitta F (1995) Non-radioactive localization of nucleic acids by direct in situ PCR and in situ RT-PCR in paraffin-embedded sections. J Histochem Cytochem 43: 739–747.
Kellogg DE, Rybalkin I, Chen S, Mukhamedova N, Vlasik T, Siebert PD, Chenchik A (1994) TaqStart antibody; “hot start” PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 6: 1134–1137.
O’Leary JJ, Browne G, Landers RJ, Crowley M, Bailey Healy I, Street JT, Pollock AM, Murphy J, Johnson MI, Lewis FA, et al. (1994) The importance of fixation procedures on DNA template and its suitability for solution-phase polymerase chain reaction and PCR in situ hybridization. Histochem J 26: 337–346.
Myers TW, Gelfand DH (1991) Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry 30: 7661–7663.
Macri CJ, Martinez A, Moody TW, Gray KD, Miller MJ, Gallagher M, Cuttitta F (1996) Detection of adrenomedullin, a hypotensive peptide, in amniotic fluid and fetal membranes. Am J Obstet Gynecol 175: 906–911.
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Martínez, A. (1998). Application of In Situ PCR and In Situ Hybridization to the Characterization of Lung Cancers. In: Martinet, Y., Hirsch, F.R., Martinet, N., Vignaud, JM., Mulshine, J.L. (eds) Clinical and Biological Basis of Lung Cancer Prevention. Respiratory Pharmacology and Pharmacotherapy. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8924-7_6
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DOI: https://doi.org/10.1007/978-3-0348-8924-7_6
Publisher Name: Birkhäuser, Basel
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