Abstract
Human Chang liver cells continuously grown in the presence of 150 to 200 µM zinc synthesize four metallothionein (MT) isoforms differing in their primary structure (Fig. 1). The sequences of three isoforms were identical with the known hepatic forms MT-2, MT-le and MT-11 and correspond to the N-terminally acetylated gene products of the MT-2A, MT-1E and MT-1X gene, respectively [1]. The fourth isoform, designated as MT-1m, differs from MT-11 by the replacement of the conserved glutamic acid residue in position 23 with glutamine and has not yet been observed in human tissues.
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References
West AK, Stallings R, Hildebrand CE, Chiu R, Karin M, Richards RI (1990) Human metallothionein genes: structure of the functional locus at 16q13. Genomics 8: 513–518.
Cavigelli M, Kägi JHR, Hunziker PE (1993) Cell- and inducer-specific accretion of human isometallothioneins. Biochem J 292: 551–554.
Roesijadi G, Vestling MM, Murphy CM, Klerks PL, Fenselau CC (1991) Structure and time-dependent behavior of acetylated and non- acetylated forms of a molluscan metallothionein. Biochim Biophys Acta 1074: 230–236.
Studer R, Hunziker PE (1997) Quantification of basal amounts of isometallothioneins in cultured cells by reversed-phase Hplc. J Liq Chromatogr Relat Technol 20: 617–625.
Studer R, Vogt CP, Cavigelli M, Hunziker PE, Kägi JHR (1997) Metallothionein accretion in human hepatic cells is linked to cellular proliferation. Biochem J 328: 63–67.
Ohtake H, Hasegawa K, Koga M (1978) Zinc-binding protein in the livers of neonatal, normal and partially hepatectomized rats. Biochem J 174: 999–1005.
Cain K, Griffiths BL (1984) A comparison of isometallothionein synthesis in rat liver after partial hepatectomy and parenteral zinc injection. Biochem J 217: 85–92.
Tohyama C, Suzuki JS, Hemelraad J, Nishimura NNH (1993) Induction of metallothionein and its localization in the nucleus of rat hepatocytes after partial hepatectomy. Hepatology 18: 1193–1201.
Nishimura H, Nishimura N, Tohyama C (1989) Immunohistochemical localization of metallothionein in developing rat tissues. J Histochem Cytochem 37: 715–722.
Nagel WW, Vallee BL (1995) Cell cycle regulation of metallothionein in human colonic cancer cells. Proc Natl Acad Sci USA 92: 579–583.
Meister A, Anderson ME (1983) Glutathione. Annu Rev Biochem 52: 711–760.
Rabenstein DL (1989) In: D Dolphin, R Poulsen, O Avramovic (eds): Glutathione: Chemical, Biochemical and Medical Aspects. John Wiley and Sons, New York, NY, pp. 147–186.
Christie NT, Costa M (1984) In vitro assessment of the toxicity of metal compuonds: IV. Disposition of metals in cells: Interactions with membranes, glutathione, metallothionein, and DNA. Biol Trace Elem Res 6: 139–158.
Ferreira AM, Ciriolo MR, Marcocci L, Rotilio G (1993) Copper(I) transfer into metallothionein mediated by glutathione. Biochem J 292: 673–676.
Perrin DD, Watt AE (1971) Complex formation of zinc and cadmium with glutathione. Biochim Biophys Acta 230: 96–104.
Duthie SJ, Collins AR (1997) The influence of cell growth, detoxifying enzymes and DNA repair on hydrogen peroxide-mediated DNA damage (measured using the comet assay) in human cells. Free Radical Biol Med 22: 717–724.
Porreca E, Di Febbo C, Pandolfi A, D’Orazio A, Martelli N, Mezzetti A, Cuccurullo F, Poggi A (1993) Differences in the glutathione system of cultured aortic smooth muscle cells from young and aged rats. Atherosclerosis 100: 141–148.
Hess D, Studer R, Hunziker PE (1994) Identification and characterization of metal protein complexes of crude cell extracts by mass-spectrometry. J Prot Chem 13: 531.
Hunziker PE, Kaur P, Wan M, Kanzig A (1995) Primary structures of seven metallothioneins from rabbit tissue. Biochem J 306: 265–270.
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Willi, A., Hunziker, P.E. (1999). Characterization of isometallothioneins in Chang liver cells and the transient accumulation of metallothionein and glutathione during cell proliferation. In: Klaassen, C.D. (eds) Metallothionein IV. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8847-9_15
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DOI: https://doi.org/10.1007/978-3-0348-8847-9_15
Publisher Name: Birkhäuser, Basel
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