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Quantification and imaging of vascular adhesion molecule expression in inflammatory diseases in vivo

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Vascular Adhesion Molecules and Inflammation

Part of the book series: Progress in Inflammation Research ((PIR))

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Abstract

Advances in our understanding of the molecular mechanisms involved in leukocyte-endothelial cell interactions have suggested novel methods for quantifying and imaging endothelial cell activation in vivo [1, 2]. Activated endothelial cells express several molecules related to leukocyte recruitment that provide possible targets [3]. Molecular probes to these might be clinically useful for imaging inflammation and provide more readily interpretable information than that supplied by existing techniques [4]. In this chapter we describe our experience evaluating E-selectin and vascular cell adhesion molecule-1 (VCAM-1) for this purpose.

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References

  1. De Bois MHW, Pauwels EKJ, Breedveld FC (1995) New agents for scintigraphy in rheumatoid arthritis. Eur J Nucl Med 22: 1339–1346

    Article  PubMed  Google Scholar 

  2. Becker W (1995) The contribution of nuclear medicine to the patient with infection. Eur J Nucl Med 22: 1195–1211

    Article  PubMed  CAS  Google Scholar 

  3. Pober JS, Cotran RS (1990) Cytokines and endothelial cell biology. Physiol Rev 70: 427–451

    PubMed  CAS  Google Scholar 

  4. Peters AM (1996) The choice of an appropriate agent for imaging inflammation. Nucl Med Commun 17: 455–458

    Article  PubMed  CAS  Google Scholar 

  5. Pober JS, Bevilacqua MP, Mendrick DL, Lapierre LA, Fiers W, Gimbrone MA (1986) Two distinct monokines, interleukin 1 and tumor necrosis factor, each independently induce the biosynthesis and transient expression of the same antigen on the surface of cultured human vascular endothelial cells. J Immunol 136: 1680–1687

    PubMed  CAS  Google Scholar 

  6. Wellicome SM, Thornhill MH, Pitzalis C, Thomas DS, Lanchbury JSS, Panayi GS, Haskard DO (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1 or lipopolysaccharide. J Immunol 144: 2558–2565

    PubMed  CAS  Google Scholar 

  7. Tsang Y, Stevens PE, Licence ST, Haskard DO, Binns RM, Robinson MK (1995) Porcine E-selectin: cloning and functional characterization. Immunology 85: 140–145

    PubMed  CAS  Google Scholar 

  8. Mason JC, Haskard DO (1994) The clinical importance of leukocyte and endothelial cell adhesion molecules in inflammation. Vasc Med Rev 5: 249–275

    Google Scholar 

  9. von Asmuth EJU, Smeets EF, Ginsel LA, Onderwater JJM, Leeuwenberg JFM, Buurman WA (1992) Evidence for endocytosis of E-selectin in human endothelial cells. Eur J Immunol 22: 2519–2526

    Article  Google Scholar 

  10. Kuijpers TW, Raleigh M, Kavanagh T, Janssen H, Calafat J, Roos D, Harlan JM (1994) Cytokine-activated endothelial cells internalize E-selectin into a lysosomal compartment of vesiculotubular shape: a tubulin-driven process. J Immunol 152: 5060–5069

    PubMed  CAS  Google Scholar 

  11. Rice GE, Bevilacqua MP (1989) An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science 246: 1303–1306

    Article  PubMed  CAS  Google Scholar 

  12. Osborn L, Hession C, Tizard R, Vassallo C, Luhowskyj S, Chi-Rosso G, Lobb R (1989) Direct expression cloning of vascular cell adhesion molecule 1 (VCAM1), a cytokine-induced endothelial protein that binds to lymphocytes. Cell 59: 1203–1211

    Article  PubMed  CAS  Google Scholar 

  13. Thornhill MH, Haskard DO (1990) IL-4 regulates endothelial activation by IL-1, tumor necrosis factor or IFNy. J Immunol 145: 865–872

    PubMed  CAS  Google Scholar 

  14. Swerlick RA, Lee KH, Li L-J, Sepp NT, Caughman SW, Lawley TJ (1992) Regulation of vascular cell adhesion molecule 1 on human dermal microvascular endothelial cells. J Immunol 149: 698–705

    PubMed  CAS  Google Scholar 

  15. Gille J, Swerlick RA, Lawley TJ, Caughman SW (1996) Differential regulation of vascular cell adhesion molecule-1 gene transcription by tumour necrosis factor alpha and interleukin-1 alpha in dermal microvascular endothelial cells. Blood 87: 211–217

    PubMed  CAS  Google Scholar 

  16. Rice GE, Munro JM, Corless C, Bevilacqua MP (1991) Vascular and nonvascular expression of INCAM-110: A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. Am J Pathol 138: 385–393

    PubMed  CAS  Google Scholar 

  17. Leeuwenberg JFM, Smeets EF, Neefjes JJ, Shaffer MA, Cinek T, Jeunhomme TMAA, Ahern TJ, Buurman WA (1992) E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77: 543–549

    PubMed  CAS  Google Scholar 

  18. Binns RM, Licence ST, Wooding ST (1990) Phytohemagglutinin induces major short-term protease-sensitive lymphocyte traffic involving high endothelium venule-like blood vessels in acute delayed-type hypersensitivity-like reactions in skin and other tissues. Eur J Immunol 20: 1067–1071

    Article  PubMed  CAS  Google Scholar 

  19. Binns RM, Licence ST, Wooding FBP, Duffus WPH (1992) Active lymphocyte traffic induced in the periphery by cytokines and phytohemagglutinin: three different mechanisms? Eur J Immunol 22: 2195–2203

    CAS  Google Scholar 

  20. Binns RM, Pabst R (1994) Lymphoid tissue structure and lymphocyte trafficking in the pig. Vet Immunol Immunopathol 43: 79–87

    Article  PubMed  CAS  Google Scholar 

  21. Harrison AA, Stocker CJ, Chapman PT, Tsang YT, Huehns TY, Gundel RH, Peters AM, Davies KA, George AJ, Robinson MK et al (1997) Expression of VCAM-1 by vascular endothelial cells in immune-and non-immune inflammatory reactions in the skin. J Immunol 159: 4546–4554

    PubMed  CAS  Google Scholar 

  22. Keelan ETM, Licence ST, Peters AM, Binns RM, Haskard DO (1994) Characterization of E-selectin expression in vivo using a radiolabelled monoclonal antibody. Am Phys-iol 266: H279–H290

    CAS  Google Scholar 

  23. Jamar F, Chapman PT, Harrison AA, Binns RM, Haskard DO, Peters AM (1995) Inflammatory arthritis: imaging of endothelial activation with an indium-111-labeled F(ab’)2 fragment of anti-E-selectin monoclonal antibody. Radiology 194: 843–850

    PubMed  CAS  Google Scholar 

  24. Gearing AJH, Newman W (1993) Circulating adhesion molecules in disease. Immunology Today 14: 506–512

    Article  PubMed  CAS  Google Scholar 

  25. Binns RM, Licence ST, Harrison AA, Keelan ETD, Robinson MK, Haskard DO (1966) In vivo E-selectin upregulation correlates with early infiltration of PMN, later with PBL-entry; mAbs block both. Am J Physiol 270: H183–H193

    Google Scholar 

  26. Keelan ETM, Harrison AA, Chapman PT, Binns RM, Peters AM, Haskard DO (1994) Imaging vascular endothelial activation: an approach using radiolabelled monoclonal antibody against the endothelial cell adhesion molecule E-selectin. J Nucl Med 35: 276–281

    PubMed  CAS  Google Scholar 

  27. Chapman PT, Jamar F, Harrison AA, Binns RM, Peters AM, Haskard DO. (1994) Non-invasive imaging of E-selectin expression by activated endothelium in urate crystal-induced arthritis. Arthritis Rheum 37: 1752–1756

    Article  PubMed  CAS  Google Scholar 

  28. Chapman PT, Jamar F, Keelan ETM, Peters AM, Haskard DO (1966) Use of a radiola-beled monoclonal antibody against E-selectin for imaging endothelial activation in rheumatoid arthritis. Arthritis Rheum 39: 1371–1375

    Article  Google Scholar 

  29. Scholmerich J, Schmidt E, Schumichen C, Billmann P, Schmidt H, Gerok W (1988) Scintigraphic assessment of bowel involvement and disease activity in Crohn’s disease using technetium 99m-hexamethyl propylene amine oxime as leukocyte label. Gas-troenterology 95: 1287–1293

    CAS  Google Scholar 

  30. Peters AM, Danpure HJ, Osman S, Hawker RJ, Henderson BL, Hodgson HJ, Kelly JD, Neirinckx RD, Lavender JP (1986) Clinical experience with 99m Tc hexamethylpropylene amine oxime for labelling leucocytes and imaging inflammation. Lancet 2: 946–949

    Article  PubMed  CAS  Google Scholar 

  31. Ohtani H, Nakamura S, Watanabe Y, Fukushima K, Mizoi T, Kimura M, Hiwatashi N, Nagura H (1992) Light and electron microscopic immunolocalization of endothelial leucocyte adhesion molecule-1 in inflammatory bowel disease. Morphological evidence of active synthesis and secretion into vascular lumen. Virchows Arch A Pathol Anat Histopathol 420: 403–409

    Article  PubMed  CAS  Google Scholar 

  32. Koizumi M, King N, Lobb R, Benjamin C, Podolsky DK (1992) Expression of vascular adhesion molecules in inflammatory bowel disease. Gastroenterology 103: 840–847

    PubMed  CAS  Google Scholar 

  33. Bhatti M, Chapman P, Peters AM, Haskard DO, Hodgson H (1998) Visualizing E-selectin in the detection and evaluation of inflammatory bowel disease. Gut 43: 40–47

    Article  PubMed  CAS  Google Scholar 

  34. Panes J, Perry MA, Anderson DC, Manning A, Leone B, Cepinskas G, Rosenbloom CL, Miyasaka M, Kvietys PR, Granger DN (1995) Regional differences in constitutive and induced ICAM-1 expression in vivo. Am J Physiol 38: H1955–H1964

    Google Scholar 

  35. Eppihimer MJ, Wolitzky BA, Anderson DC, Labow MA, Granger DN (1996) Heterogeneity of expression of E-and P-selectins in vivo. Circ Res 79: 560–569

    Article  PubMed  CAS  Google Scholar 

  36. Henninger DD, Panes J, Eppihimer M, Russell J, Gerritsen M, Anderson DC, Granger DN (1997) Cytokine-induced VCAM-1 and ICAM-1 expression in different organs in the mouse. J Immunol 158: 1825–1832

    PubMed  CAS  Google Scholar 

  37. Wickham TJ, Haskard D, Segal D, Kovesdi I (1997) Targeting endothelium for gene therapy via receptors upregulated during angiogenesis and inflammation. Cancer Immunol Immunother 45: 149–151

    Article  PubMed  CAS  Google Scholar 

  38. Harari OA, Wickham TJ, Stocker CJ, Kovesdi I, Segal DM, Huehns TY, Sarraf C, Haskard DO (1999) Targeting an adenoviral gene vector to cytokine-activated vascular endothelium via E-selectin. Gene Therapy 5: 801–807

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. BHF Cardiovascular Medicine Unit, National Heart and Lung Institute (Hammersmith Hospital), Imperial College School of Medicine, Du Cane Road, London, W12 ONN, UK

    Tanya Y. Huehns & Dorian O. Haskard

Authors
  1. Tanya Y. Huehns
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  2. Dorian O. Haskard
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Editor information

Editors and Affiliations

  1. Centre for Cardiovascular Biology and Medicine, School of Biomedical Sciences King’s College London, Guy’s Campus, London, SE1 9RT, UK

    Jeremy D. Pearson

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© 1999 Springer Basel AG

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Huehns, T.Y., Haskard, D.O. (1999). Quantification and imaging of vascular adhesion molecule expression in inflammatory diseases in vivo . In: Pearson, J.D. (eds) Vascular Adhesion Molecules and Inflammation. Progress in Inflammation Research. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8743-4_10

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  • DOI: https://doi.org/10.1007/978-3-0348-8743-4_10

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9753-2

  • Online ISBN: 978-3-0348-8743-4

  • eBook Packages: Springer Book Archive

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