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Use of the Xenopus Oocyte System to Study RNA Transport

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Microinjection

Part of the book series: Methods and Tools in Biosciences and Medicine ((MTBM))

Abstract

Xenopus oocytes have been used extensively in studies of RNA nuclear transport. They are readily obtained from adult females, are large enough to be easily manipulated and microinjected, and the nucleus can be manually dissected from the oocyte (Fig. 1), allowing a simple determination of nuclear transport by measurement of the cytoplasmic and nuclear pools of the RNA under study.

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© 1999 Springer Basel AG

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Allison, L.A. (1999). Use of the Xenopus Oocyte System to Study RNA Transport. In: Lacal, J.C., Feramisco, J., Perona, R. (eds) Microinjection. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8705-2_15

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  • DOI: https://doi.org/10.1007/978-3-0348-8705-2_15

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-7643-6019-1

  • Online ISBN: 978-3-0348-8705-2

  • eBook Packages: Springer Book Archive

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