Summary
DNA probes which detect polymorphic, repetitive sequences in a variety of genomes have been developed using different approaches. Naturally occurring plasmids, with repeated units termed iterons near or within their origins (ori) of replication, could be of interest for the development of probes, possibly even revealing novel minisatellite families in mammals involved in replicational processes. We used the plasmids P1, pSC101 and RSF1010 or their PCR amplified ori regions as probes in Southern blot hybridisations with mammalian DNA. At low stringency they generated reproducible fingerprint-like patterns. A bovine genomic library was screened at the same stringency with the PCR-amplified ori region of PI containing the five times repeated core sequence 5’-ATGTGTGNTGNNGGG-3’ to generate a probe for cattle DNA with higher specificity.
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References
Ali S, Müller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotide probes specific for simple repeats. Hum Genet 74: 239–243
Birnboim HC, Doly J (1979) A rapid akaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7: 1513–1523
Collick A, Jeffreys AJ (1990) Detection of a novel minisatellite specific DNA-binding protein. Nucleic Acids Res 18: 625–629
Crawford AM, Buchanan FC, Fraser KM, Robinson AJ, Hill DF (1991) Repeat sequences from complex ds viruses can be used as minisatellite probes for DNA fingerprinting. Animal Genetics 22: 177–181
Frey J, Bagdasarian M (1989). The molecular biology of IncQ plasmids. In: Thomas CM (ed) Promiscuous Plasmids of Gram-negative Bacteria. Academic Press, London: 79–94
Jeanpierre M (1987) A rapid method for the purification of DNA from blood. Nucleic Acids Res 15: 9611
Jeffreys AJ, Wilson V, Thein SL (1985) Hypervariable minisatellite regions in human DNA. Nature 314: 67–73
Kaukine J and Varvio S-L (1992) Artiodactyl retroposons: association with microsatellites and use in SINEmorph detection by PCR. Nucleic Acids Res 20: 2955–2958
Linder P, Churchward G, Guixian X, Yi-Yi Y, Caro L (1985) An essential replication gene, repA, of plasmid pSC101 is autoregulated. J Mol Biol 181: 383–393
Nakamura Y, Leppert M, O’Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E and White R (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616–1622
Novick RP (1987) Plasmid Incompatibility. Microbiol Rev 51: 381–395
Persson C and Nordström K (1986) Control of replication of the broad host range plasmid RSF 1010: The incompatibility determinant consists of directly repeated DNA sequences. Mol Gen Genet 203: 189–192
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Sanger F, Nikeln S, Coulson AR (1977) DNA sequencing with chaintermination inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Scott J (1984) Regulation of plasmid replication. Microbiol Rev 48: 2–23
Vassart G, Georges M, Monsieur EH (1987) Hypervariable minisatellites in human and animal DNA. Science 235: 683–684
Vergnaud G (1989) Polymers or random short oligonucleotides detect polymorphic loci in the human genome. Nucleic Acids Res 19: 7623–7630
Vieira J, Messing J (1982) The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259–268
Weber JL (1990) Informativeness of human (dC-dA)n × (dG — dT)n polymorphisms. Genomics 7: 524–530
Westneat DF, Noon WA, Reeve HK and Aquadro CF (1988) Improved hybridization conditions for DNA “fingerprints ”probed with M13. Nucleic Acids Res NAC 16: 4161
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© 1993 Springer Basel AG
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Huebscher, K.J., Dolf, G., Frey, J. (1993). Iterons of stringently controlled plasmids and DNA fingerprinting. In: Pena, S.D.J., Chakraborty, R., Epplen, J.T., Jeffreys, A.J. (eds) DNA Fingerprinting: State of the Science. Progress in Systems and Control Theory. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8583-6_8
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DOI: https://doi.org/10.1007/978-3-0348-8583-6_8
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-7643-2906-8
Online ISBN: 978-3-0348-8583-6
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