Summary
Sperm utilization by multiply inseminated females, such as honeybee queens, is of key importance because of its relationship to kin selection theory and the evolution of eusociality. We have used M13 phage DNA fingerprinting analysis to study the number of drones that contribute patrilines to an Apis mellifera ligustica colony in Argentina. Ten different paternal patterns were clearly distinguished in a 28 worker offspring of this naturally inseminated queen. Relative paternal contributions ranged from 3.5% to 25%. These results do not seem to adjust to random sperm use nor do they support sperm clumping. M13 phage DNA fingerprinting has proven to be an adequate tool for sperm use studies in this species.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Blanchetot A (1991) Genetic relatedness in honeybees as established by DNA fingerprinting. J Hered 82: 391–396
Corley J, Rabinovich M, Seigelchifer M, Zorzopulos J, Corley E (1992) DNA fingerprinting in honeybees. Fingerprint News 4(2): 8–11
Feinberg AP, Vogelstein BA (1983) Technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13
Hall G (1991) Genetic characterization of honeybees through DNA analysis. In: Spivak M, Fletcher DJC, Breed MD (eds) The “African” Honeybee. Westview press, Boulder, Colorado, pp 45–73
Jeffreys AJ, Turner M, Debenham P (1991) The efficiency of multilocus DNA fingerprint probes for individualization and establishment of family relationships, determined from extensive casework. Am J Hum Genet 48: 824–840
Laidlaw HH, Page RE (1984) Polyandry in honey bees (Apis mellifera L.): Sperm utilization and intracolony genetic relationships. Genetics 108: 985–997
Moritz RFA, Meusel MS, Harberl M (1991) Oligonucleotide DNA fingerprinting discriminates super-and half-sisters in honeybee colonies (Apis mellifera L.). Naturwissenschaften 78: 422–424
Page RE (1986) Sperm utilization in social insects. Ann Rev Entomol 31: 297–320
Seeley TD (1985) Honeybee ecology. Princeton University press, Princeton, New Jersey
Tucker K (1958) Automictic parthogenesis in the honey bee. Genetics 43: 299–316
Vassart G, Georges M, Monsieur R, Brocas H, Lequarré AS, Christophe DA (1987) Sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235: 683–684
Weihe A, Niemann C, Lieckfield D, Meyer W, Borner T (1990) An improved hybridization procedure for DNA fingerprinting with bacteriophage M13 as a probe. Fingerprint News 2(4): 9–10
Westneat DF, Noon WA, Reeve HK, Aquadro CF (1988) Improved hybridization conditions for DNA fingerprints probed with M13. Nucleic Acids Res 16: 4161
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer Basel AG
About this chapter
Cite this chapter
Corley, J., Rabinovich, M., Seigelchifer, M., Corley, E., ZorzÓpulos, J. (1993). Sperm utilization in honeybees as detected by M13 DNA fingerprints. In: Pena, S.D.J., Chakraborty, R., Epplen, J.T., Jeffreys, A.J. (eds) DNA Fingerprinting: State of the Science. Progress in Systems and Control Theory. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8583-6_33
Download citation
DOI: https://doi.org/10.1007/978-3-0348-8583-6_33
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-7643-2906-8
Online ISBN: 978-3-0348-8583-6
eBook Packages: Springer Book Archive