Skip to main content
SpringerLink
Log in

Characterization of arbuscular mycorrhizal fungi by immunochemical methods

  • Chapter
Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems

Part of the book series: ALS Advances in Life Sciences ((ALS))

Abstract

The application of antibodies (Ab) as molecular probes is based upon their highly selective binding to corresponding antigens. There is a long tradition for the use of immunochemical techniques in microbiology, and although emphasis has been mainly placed on bacteria and viruses, there is an increasing number of examples where Ab have been raised against fungi (Hardham et al., 1986; Straker et al., 1989; Dewey, 1990; Breuil et al., 1992; De Ruiter et al., 1993). In the field of arbuscular mycorrhizal fungi (AMF) immunochemistry represents an extremely useful approach for research into systematic, ultrastructural and ecological aspects pertaining to the fungi forming these highly complex symbioses.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Aldwell, F. E. B. and Hall, I. R. (1986) Monitoring spread of Glomus mosseae through soil infested with Acaulospora laevis using serological and morphological techniques. Trans. Br. Mycol. Soc. 87:131–134.

    Article  Google Scholar 

  • Aldwell, F. E. B., Hall, I. R. and Smith, J. M. B. (1983) Enzyme-linked immunosorbent assay (ELISA) to identify endomycorrhizal fungi. Soil Biol. Biochem. 15: 377–378.

    Article  Google Scholar 

  • Aldwell, F. E. B., Hall, I. R. and Smith, J. M. B. (1985) Enzyme-linked immunosorbent assay as an aid to taxonomy of the Endogonaceae. Trans. Br. Mycol. Soc. 84: 399–402.

    Article  Google Scholar 

  • Bonfante-Fasolo, P., Faccio, A., Perotto, S. and Schubert, A. (1990) Correlation between chitin distribution and cell wall morphology in the mycorrhizal fungus Glomus versiformt. Mycol. Res. 94: 157–165.

    Article  CAS  Google Scholar 

  • Breuil, C., Luck, B. T., Rossignol, L., Little, J., Echeverri, C. J., Banerjee, S. and Brown, D. L. (1992) Monoclonal antibodies to Gliocladium roseum, a potential biological control fungus of sap-staining fungi in wood. J. Gen. Microbiol. 138: 2311–2319.

    PubMed  CAS  Google Scholar 

  • Cordier, C., Gianinazzi-Pearson, V. and Gianinazzi, S. (1994) Immunodétection de champignons endomycorhiziens à arbuscules in planta. Acta Bot. Gallica (in press).

    Google Scholar 

  • De Ruiter, G. A., van Bruggen-van der Lugt, A. W., Bos, W., Notermans, S. H. W., Rombouts, F. M. and Hofstra, H. (1993) The production and partial characterization of a monoclonal IgG antibody specific for moulds belonging to the order Mucorales. J. Gen. Microbiol. 139:1557–1564.

    PubMed  Google Scholar 

  • Dewey, F. M. (1990) The use of monoclonal antibodies to detect plant invading fungi. In: A. Schots (ed) Monoclonal Antibodies in Agriculture-Proceedings of the Symposium “Perspectives for Monoclonal Antibodies in Agriculture”. Laboratorium for Monoclonal Antibodies, Wageningen, Niederlande-Pudoc, pp 21–25.

    Google Scholar 

  • Friese, C. F. and Allen, M. F. (1991) Tracking the fates of exotic and local VA mycorrhizal fungi: methods and patterns. Agric. Ecosys. Environ. 34: 87–96.

    Article  Google Scholar 

  • Gianinazzi, S. and Gianinazzi-Pearson, V. (1992) Cytology, histochemistry and immunocytochemistry as tools for studying structure and function in endomycorrhizas. In: J.R. Norris, D.J. Read and A.K. Varma (eds) Methods in Microbiology 24: 109–139.

    Google Scholar 

  • Gianinazzi-Pearson, V. and Gianinazzi, S. (1978) Enzymatic studies on the metabolism of vesicular-arbuscular mycorrhiza. II soluble alkaline phosphatase specific to the mycorrhizal infection in onion roots. Physiol. Plant Pathol. 12: 45–53.

    Article  CAS  Google Scholar 

  • Gianinazzi-Pearson, V. and Gianinazzi, S. (1994) Proteins and protein activities in endomycorrhizal symbioses. In: B. Hock and A.K. Varma (eds) Mycorrhiza: Function, Molecular Biology and Biotechnology. Springer, Heidelberg (in press).

    Google Scholar 

  • Gianinazzi-Pearson, V., Lemoine, M. C., Arnould, C. and Morton, J. B. (1994) Localization of ß(1–3) glucans in spore and hyphal walls of fungi in the Glomales: taxonomic and phylogenetic implications. Mycologia (in press).

    Google Scholar 

  • Hahn, A., Bonfante, P., Horn, K., Pausch, F. and Hock, B. (1993) Production of monoclonal antibodies against surface antigens of spores from arbuscular mycorrhizal fungi by an improved immunization and screening procedure. Mycorrhiza 4:69–78.

    Article  CAS  Google Scholar 

  • Hahn, A., Horn, K. and Hock, B. (1994) Serological properties of mycorrhizae. In: B. Hock and A.K. Varma (eds) Mycorrhiza: Function, Molecular Biology and Biotechnology. Springer-Verlag, Heidelberg, in press.

    Google Scholar 

  • Hardham, A. R., Suzaki, E. and Perkin, J. L. (1986) Monoclonal antibodies to isolate-, species-, and genus-specific components on the surface of zoospores and cysts of the fungus Phytophthora cimmamomi. Can. J. Bot. 64: 311–321.

    Article  Google Scholar 

  • Harlow, E. and Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbour Laboratory, New York.

    Google Scholar 

  • Köhler, G. and Milstein, C, (1975) Continuous cultures of fused cells secreting antibody of predefined specifity. Nature 256: 495–497.

    Article  PubMed  Google Scholar 

  • Kough, J., Malajczuk, N. and Linderman, R. G. (1983) Use of the indirect immunofluorescent technique to study the vesicular-arbuscular fungus Glomus epigaeum and other Glomus species. New Phytol. 94: 57–62.

    Article  Google Scholar 

  • Mernaugh, R. L., Mernaugh, G. R. and Kovacs G R (1990) The immune response: antigens, antibodies, antigen-antibody interactions. In: R. Hampton, E. Ball and S. De Boer (ed) Immunochemical Methods for the Detection of Viral and Bacterial Plant Pathogens. APS Press, St. Paul, pp 3–14.

    Google Scholar 

  • Morton, J. B. (1988) Taxonomy of VA mycorrhizal fungi: classification, nomenclature, and identification. Mycotaxon 32: 267–324.

    Google Scholar 

  • Morton, J. B. (1990) Evolutionary relationships among arbuscular mycorrhizal fungi in the Endogonaceae. Mycologia 82: 192–207.

    Article  Google Scholar 

  • Oramas-Shirey, M. and Morton, J. B. (1990) Immunological stability among different geographic isolates of the arbuscular mycorrhizal fungus Glomus occultum. Abstracts of the 90th Annual Meeting of the American Society for Microbiology: 311 (Q-138).

    Google Scholar 

  • Ravolanirina, F. and Gianinazzi-Pearson, V. (1992) VA endomycorrhization of microplants of grapevine: an immunological approach. In: J.N. Wolf (ed) Interactions between Plants and Microorganisms. Regional Seminar, International Foundation for Science, Stockholm, pp. 186–198.

    Google Scholar 

  • Roitt, I. M. (1991) Essential immunology. 7th ed., Blackwell Scientific Publ, Oxford.

    Google Scholar 

  • Sanders, I. R., Ravolanirina, F., Gianinazzi-Pearson, V., Gianinazzi, S. and Lemoine, M. C. (1992) Detection of specific antigens in the vesicular-arbuscular mycorrhizal fungi Gigaspora margarita and Acaulospora laevis using polyclonal antibodies to soluble spore fractions. Mycol. Res. 96: 477–480.

    Article  Google Scholar 

  • Simon, L, Bousquet, J., Lévesque, C. and Lalonde, M. (1993) Origin and diversification of endomycorrhizal fungi and coincidence with vascular land plants. Nature 363:67–69

    Article  Google Scholar 

  • Straker, C. J., Gianinazzi-Pearson, V., Gianinazzi, S., Cleyet-Marel, J. C. and Bousquet, N. (1989) Electrophoretic and immunological studies on acid phosphatase from a mycorrhizal fungus of Erica hispidula. New Phytol. 111: 215–221.

    Article  CAS  Google Scholar 

  • Tisserant, B. (1993) VA endomycorrhization of woody plants: root architecture and functional activity of the endomycorrhizal symbiosis. Thesis, University of Dijon, F: 136 pages.

    Google Scholar 

  • Tisserant, B., Gianinazzi-Pearson, V., Gianinazzi, S. and Gollotte, A. (1993) In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infection. Mycol Res. 97: 245–250.

    Article  CAS  Google Scholar 

  • Weinbaum, B. S., Allen, M. F. and Friese, C. F. (1992) Tracking the fate of Acaulospora and Scutellospora using fluorescent antibodies. Newsletter of the MSA 43: 54.

    Google Scholar 

  • Wilson, I. M., Trinick, M. J. and Parker, C. A. (1983) The identification of vesicular-arbuscular mycorrhizal fungi using immunofluorescence. Soil Biol. Biochem. 15: 439–445.

    Article  Google Scholar 

  • Wright, S. F. and Morton, J. B. (1989) Detection of vesicular-arbuscular mycorrhizal fungus colonization of roots by using a dot-immunoblot assay. Appl. Environm. Microbiol. 55: 761–763.

    CAS  Google Scholar 

  • Wright, S. F., Morton, J. B. and Sworobuk, J. E. (1987) Identification of a vesicular-arbuscular mycorrhizal fungus by using monoclonal antibodies in an enzyme-linked immunosorbent assay. Appl. Environ. Microbiology 53: 2222–2225.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Technische Universität München (Weihenstephan), Lehrstuhl für Botanik, D-85350, Freising, Germany

    A. Hahn & B. Hock

  2. Laboratoire de Phytoparasitologie, INRA-CNRS, Station de Génétique et d’Amélioration des Plantes, INRA, BV 1540, F-21034, Dijon cédex, France

    V. Gianinazzi-Pearson

Authors
  1. A. Hahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Gianinazzi-Pearson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Hock
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratoire de Phytoparasitologie, INRA/CNRS, SGAP, INRA, BV 1540, F - 21034, Dijon Cédex, France

    S. Gianinazzi

  2. Eidgenössische Forschungsanstalt für Obst-, Wein- und Gartenanbau, CH-8820, Wädenswil, Switzerland

    H. Schüepp

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Springer Basel AG

About this chapter

Cite this chapter

Hahn, A., Gianinazzi-Pearson, V., Hock, B. (1994). Characterization of arbuscular mycorrhizal fungi by immunochemical methods. In: Gianinazzi, S., Schüepp, H. (eds) Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems. ALS Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8504-1_3

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-8504-1_3

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9654-2

  • Online ISBN: 978-3-0348-8504-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation