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Evolving measurements of radical products — how much do they tell us about inflammation?

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Free Radicals and Inflammation

Part of the book series: Progress in Inflammation Research ((PIR))

Abstract

It is now widely accepted that reactive oxygen species (ROS) such as superoxide radical (O2 ·-), hydrogen peroxide (H2O2) and hydroxyl radical (•OH) reactive chlorine species (RCS) such as hypochlorous acid (HOCl) and reactive nitrogen species (RNS) such as nitrogen monoxide (nitric oxide, NO>•) and oxoperoxonitrate (peroxynitrite, ONOO-) contribute to considerable tissue injury during chronic inflamation (reviewed in [1]).

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References

  1. Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine, 3rd ed. Oxford University Press, UK

    Google Scholar 

  2. Moncada S, Higgs (1993) The L-arginine-nitric oxide pathway. N Engl J Med 329: 2002–2011

    Article  PubMed  CAS  Google Scholar 

  3. Grisham MB (1994) Oxidants and free radicals in inflammatory bowl disease. Lancet 344: 859–861

    Article  PubMed  CAS  Google Scholar 

  4. Anggard E (1994) Nitric oxide: Mediator, murderer and medicine. Lancet 343: 1199–1206

    Article  PubMed  CAS  Google Scholar 

  5. Babior BM, Woodman RC (1990) Chronic granulomatous disease. Sem Hematol 27: 247–259

    CAS  Google Scholar 

  6. Weiss SJ (1989) Tissue destruction by neutrophils. N Engl J Med 320: 365–376

    Article  PubMed  CAS  Google Scholar 

  7. Rowley DA, Gutteridge JMC, Blake DR, Farr M, Halliwell B (1984) Lipid peroxidation in rheumatoid arthritis: thiobarbituric-reactive material and catalytic iron salts in synovial fluid from rheumatoid patients. Clin Sci 66: 691–695

    PubMed  CAS  Google Scholar 

  8. Humad S, Zarling E, Clapper M and Skosey JL (1988) Breath pentane excretion as a marker of disease activity in rheumatoid arthritis. Free Rad Res Commun 5: 101–106

    Article  CAS  Google Scholar 

  9. Lunec J, Blake DR, McCleary SJ, Brailford S, Bacon PA (1985) Self-perpetuating mechanisms of immunoglobulin G aggregation in rheumatoid inflammation. J Clin Invest 76: 2084–2090

    Article  PubMed  CAS  Google Scholar 

  10. Griffiths HR, Lunec J (1991) Effects of reactive oxygen species on immunoglobulinG function. Mol Aspects Med 12: 107–119

    Article  PubMed  CAS  Google Scholar 

  11. Lunec J, Herbert K, Blount S, Griffiths HR, Emery P (1994) 8-Hydroxydeoxyguanosine: a marker of DNA damage in systemic lupus erythematosus. FEBS Lett 348: 131–138

    Article  PubMed  CAS  Google Scholar 

  12. Bashir S, Harris G, Denman MA, Blake DR, Winyard PG (1993) Oxidative damage and cellular sensitivity to oxidative stress in human autoimmune diseases. Ann Rheum Dis 52: 659–666

    Article  PubMed  CAS  Google Scholar 

  13. Grootveld M, Halliwell B (1987) Measurement of allantoin and uric acid in human body fluids. A potential index of free radical reactions in vivo. Biochem J 243: 803–808.

    CAS  Google Scholar 

  14. Grootveld M, Henderson EB, Farrell A, Blake DR, Parkes HG, Haycock P (1991) Oxidative damage to hyaluronate and glucose in synovial fluid during exercise of the inflamed rheumatoid joint. Biochem J 273: 459–467

    PubMed  CAS  Google Scholar 

  15. Lunec J, Blake DR (1985) The determination of dehydroascorbic acid in the serum and synovial fluid of patients with rheumatoid arthritis. Free Rad Res Commun 1: 32–39

    Article  Google Scholar 

  16. Situnayake RD, Turnham DI, Kootathep S, Chirico S, Lunec J, Davis M, McConkey B (1991) Chain breaking antioxidant status in rheumatoid arthritis: clinical and laboratory correlates. Ann Rheum Dis 50: 81–86

    Article  PubMed  CAS  Google Scholar 

  17. Halliwell B, Gutteridge JMC (1989) Free radicals in biology and medicine. Clarendon Press, Oxford

    Google Scholar 

  18. Carr AC, van den Berg JJ, Winterbourn CC (1996) Chlorination of cholesterol in cell membranes by hypochlorous acid. Arch Biochem Biophys 332: 63–69

    Article  PubMed  CAS  Google Scholar 

  19. Beckman JS, Chen J, Ischiropoulus H, Crow JP (1994) Oxidative chemistry of peroxynitrite. Methods Enzymol 233: 229–240

    Article  PubMed  CAS  Google Scholar 

  20. Pryor WA, Squadrito GL (1995) The chemistry of peroxinitrite — a product from the reaction of nitric oxide with superoxide. Am J Phys 268: L699–L722

    CAS  Google Scholar 

  21. Spencer JPE, Wong J, Jenner A, Aruoma OI, Cross CE, Halliwell B (1996) Base modification and strand breakage in isolated calf thymus DNA and in DNA from human skin epidermal keratinocytes exposed to peroxynitrite or 3-morpholinosydnonimine. Chem Res Toxicol 9: 1152–1158

    Article  PubMed  CAS  Google Scholar 

  22. Sonntag von C (1987) The chemical basis of radiation biology. Taylor and Francis, London

    Google Scholar 

  23. Biemond P, Swaak AJG, Van Eijk HG, Koster JF (1986) Intraarticular ferritin-bound iron in rheumatoid arthritis. Arthritis Rheum 29: 1187–1193

    Article  PubMed  CAS  Google Scholar 

  24. Halliwell B, Aruoma OI, Mufti G, Bomford A (1988) Bleomycin detectable iron in serum from leukaemic patients before and after chemotherapy. FEBS Lett 241: 202–204

    Article  PubMed  CAS  Google Scholar 

  25. Puppo A, Halliwell B (1988) Formation of hydroxyl radicals from hydrogen peroxide in the presence of iron. Is haemoglobin a biological Fenton catalyst? Biochem J 249: 185–190

    PubMed  CAS  Google Scholar 

  26. Gutteridge JMC (1987) Bleomycin-detectable iron in knee-joint synovial fluid from arthritic patients and relationships to the extracellular antioxidant activities of caeroplasmin, transferrin and lactoferrin. Biochem J 245: 415–421

    PubMed  CAS  Google Scholar 

  27. Gutteridge JMC, Rowley DA, Halliwell B (1982) Superoxide-dependent formation of hydroxyl radicals and lipid peroxidation in the presence of iron salts. Detection of catalytic iron and anti-oxidant activity in extracellular fluids. Biochem J 206: 605–609

    PubMed  CAS  Google Scholar 

  28. Winyard PG, Blake DR, Chirico S, Gutteridge JMC, Lunec J (1987) Mechanism of exacerbation of rheumatoid synovitis by total-dose iron-dextran infusion: in vivo demonstration of iron-promoted oxidative stress. Lancet I: 69–72

    Article  Google Scholar 

  29. Halliwell B, Hoult JRS, Blake DR (1988) Oxidants, inflammation, and anti-inflammatory drugs. FASEB J 2: 2867–2873

    PubMed  CAS  Google Scholar 

  30. Means JT, Krantz SB (1992) Progress in understanding the pathogenesis of the anemia of chronic disease. Blood 80: 1639–1647

    PubMed  Google Scholar 

  31. Winyard PG, Pall H, Lunec J, Blake DR (1987) Non-caeruloplasmin copper (Phenanthroline-copper) is not present in fresh serum or synovial fluid from patients with rheumatoid arthritis. Biochem J 247: 245–247

    PubMed  CAS  Google Scholar 

  32. Stevens CR, Benboubetra M, Harrison R, Sahinoglu T, Smith EC, Blake DR (1991) Location of xanthine oxidase to synovial endothelium. Ann Rheum Dis 50: 760–762

    Article  PubMed  CAS  Google Scholar 

  33. Schalkwijk J, van den Berg WB, Van de putte LBA, Joosten LAB (1986) An experimental model for hydrogen peroxide-induced tissue damage. Effect of a single inflammatory mediator on (peri) articular tissues. Arthritis Rheum 29: 532–538

    Article  PubMed  CAS  Google Scholar 

  34. Huie RE, Padmaja S (1993) The reaction of NO with superoxide. Free Rad Res Commun 18: 195–199

    Article  CAS  Google Scholar 

  35. Farrell AJ, Blake DR, Palmer RMJ, Moncada S (1992) Increased concentrations of nitrite in synovial fluid and serum samples suggest increased nitric oxide synthesis in rheumatic diseases. Ann Rheum Dis 51: 1219–1222

    Article  PubMed  CAS  Google Scholar 

  36. Merenyi G, Lind J (1997) Thermodynamics of peroxynitrite and its CO2 adduct. Chem Res Toxicol 10: 1216–1220

    Article  PubMed  CAS  Google Scholar 

  37. Kaur H, Whiteman M, Halliwell B (1997) Peroxynitrite-dependent aromatic hydroxylation and nitration of salicylate and phenylalanine. Is hydroxyl radical involved? Free Rad Res 26: 71–82

    Article  CAS  Google Scholar 

  38. Lymar SV, Jiang Q, Hurst JK (1996) Mechanism of carbon dioxide-catalysed oxidation of tyrosine by peroxynitrite. Biochemistry 35: 7855–7861

    Article  PubMed  CAS  Google Scholar 

  39. Candeias LP, Patel KB, Stratford MRL, Wardman P (1993) Free hydroxyl radicals are formed on reaction between the neutrophil derived species superoxide anion and hypochlorous acid. FEBS Lett 333: 151–153

    Article  PubMed  CAS  Google Scholar 

  40. Halliwell B (1996) Oxidative stress, nutrition and health. Experimental strategies for optimisation of nutritional antioxidants intake in humans. Free Rad Res 25: 57–74

    Article  CAS  Google Scholar 

  41. Kaur H (1996) A water soluble C-nitroso-aromatic spin-trap-3,5-dibromo-4-nitrosobenzenesulphonic acid. “The Perkins spin-trap”. Free Rad Res 24: 409–420

    Article  CAS  Google Scholar 

  42. Singh D, Nazhat NB, Fairburn K, Sahinoglu T, Blake DR, Jones P (1995) Electron spin resonance spectroscopic demonstration of the generation of reactive oxygen species by diseased human synovial tissue following ex vivo hypoxia-reoxygenation. Ann Rheum Dis 54: 94–99

    Article  PubMed  CAS  Google Scholar 

  43. Kaur H, Halliwell B (1994) Detection of hydroxyl radicals by aromatic hydroxylation. Methods Enzymol 233: 67–82

    Article  PubMed  CAS  Google Scholar 

  44. Ingelman-Sundberg M, Kaur H, Terelius Y, Persson JO, Halliwell B (1991) Hydroxylation of salicylate by microsomal fractions and cytochrome P-450. Lack of production of 2,3-dihydroxybenzoate unless hydroxyl radical formation is permitted. Biochem J 276: 757–853

    Google Scholar 

  45. Grootveld M, Halliwell B (1986) Aromatic hydroxylation as a potential measure of hydroxyl radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids. Biochem J 237: 499–5041E

    PubMed  CAS  Google Scholar 

  46. Ghiselli A, Laurenti O, Mattia GD, Mariani G, Ferro-Luzzi A (1992) Salicylate hydroxylation as an early marker of in vivo oxidative stress in diabetic patients. Free Rad Biol Med 13: 621–626

    Article  PubMed  CAS  Google Scholar 

  47. Kaur H, Edmonds SE, Blake DR, Halliwell B (1996) Hydroxyl radical generation by rheumatoid blood and knee joint synovial fluid. Ann Rheum Dis 55: 1–6

    Article  Google Scholar 

  48. Vliet van der A, Eiserich JP, Kaur H, Cross CE, Halliwell B (1996) Nitrotyrosine as biomarker for reactive nitrogen species. Methods Enzymol 269: 175–184

    Article  PubMed  Google Scholar 

  49. Kaur H, Halliwell B (1994) Evidence for nitric oxide mediated oxidative damage in chronic inflammation. FEBS Lett 350: 9–12

    Article  PubMed  CAS  Google Scholar 

  50. Kettle AJ (1996) Neutrophils convert tyrosyl residues in albumin to chlorotyrosine. FEBS Lett 379: 103–106

    Article  PubMed  CAS  Google Scholar 

  51. Eiserich JP, Cross CE, Jones DA, Halliwell B, van der Vliet A (1996) Formation of nitrating and chlorinating species by reaction of nitrite with hypochlorous acid. J Biol Chem 271: 19199–19208

    Article  PubMed  CAS  Google Scholar 

  52. Esterbauer H, Gebicki J, Puhl H and Jurgens G (1992) The role of lipid peroxidation and antioxidants in the oxidative modification of LDL. Free Rad Biol Med 13: 341–390

    Article  PubMed  CAS  Google Scholar 

  53. Halliwell B, Chirico S (1993) Lipid peroxidation: its mechanism, measurement, and significance. Am J Clin Nutr 57: 715S–725S

    PubMed  CAS  Google Scholar 

  54. Douki T, Ames BN (1994) An HPLC-EC assay for 1,N2-propano adducts of 2’deoxyguanosine with 4-hydroxynonenal and other a,(3-unsaturated aldehydes. Chem Res Toxicol 7: 511–518

    Article  PubMed  CAS  Google Scholar 

  55. Chaudary AK, Nokubo M, Reddy GR, Yeola SN, Morrow JD, Blair IA, Marnett LJ (1994) Detection of endogenous malondialdehyde-deoxyguanosine adducts in human liver. Science 265: 1580–1582

    Article  Google Scholar 

  56. Gambhir JK, Lali P, Jain AK (1997) Correlation between blood antioxidant levels and lipid peroxidation in rheumatoid arthritis. Clin Biochem 30: 351–355

    Article  PubMed  CAS  Google Scholar 

  57. Selley ML, Bourne DJ, Bartlett MR, Tymms KE, Brook AS, Duffield AM, Ardlie NG (1992) Occurrrence of (E)-4-hydroxy-2-nonenal in plasma and synovial fluid of patients with rheumatoid arthritis and osteoarthritis. Ann Rheum Dis 51: 481–484

    Article  PubMed  CAS  Google Scholar 

  58. Merry P, Grootveld M, Lunec J, Blake DR (1991) Oxidative damage to lipids within the inflamed human joint provides evidence of radical-mediated hypoxic-reperfusion injury. Am J Clin Nutr 56: 362S–369S

    Google Scholar 

  59. Morrow JD, Roberts LJ (1997) The isoprostanes: unique bioactive products of lipid per-oxidation. Prog Lipid Res 36: 1–21

    Article  PubMed  CAS  Google Scholar 

  60. Roberts LJ, Morrow JD (1996) The generation and actions of isoprostanes. Biochem Pharmacol 51: 1–9

    Article  PubMed  Google Scholar 

  61. Nourooz-Zadeh J, Halliwell B, Anggard EE (1997) Evidence for the formation of F3isoprostanes during peroxidation of eicosapentaenoic acid. Biochem Biophys Res Comm 236: 467–472

    Article  PubMed  CAS  Google Scholar 

  62. Nourooz-Zadeh J, Liu HCE, Anggard EE, Halliwell B (1997) F4-isoprostanes: A novel class of prostenoids formed during peroxidation of decosahexaenoic acid (DHA). Biochem Biophys Res Comm 242: 338–344

    Article  Google Scholar 

  63. Jira W, Spiteller G, Richter A (1997) Increased levels of lipid oxidation products in low density lipoproteins of patients suffering from rheumatoid arthritis. Chem Phys Lipids 87: 81–89

    Article  PubMed  CAS  Google Scholar 

  64. Ku G, Thomas CE, Akeson AL, Jackson RL (1992) Induction of interleukin-113 expression from peripheral blood monocyte-derived macrophages by 9-hydroxy-octadecadienoic acid. J Biol Chem 267: 14183–14188

    PubMed  CAS  Google Scholar 

  65. Olopade CO, Zakkar M, Swedler WI, Rubinstein I (1997) Exhaled pentane levels in acute asthma. Chest 111: 862–865

    Article  PubMed  CAS  Google Scholar 

  66. Kokoszka J, Nelson RL, Swedler WI, Skosey JL, Abcarian H (1993) Determination of inflammatory bowel disease activity by breath pentane analysis. Dis Colon Rectum 36: 597–601

    Article  PubMed  CAS  Google Scholar 

  67. Sedghi S, Keshavarzian A, Klamut M, Eiznhamer D, Zarling EJ (1994) Elevated breath ethane levels in active ulcerative colitis: Evidence for excessive lipid peroxidation. Am J Gastroenterol 89: 2217–2221

    CAS  Google Scholar 

  68. Kneepkens FCM (1997) Assessment of oxidative stress and antioxidant status in humans: The hydrocarbon breath test. In: Aruoma OI, Cuppett SL (eds): Antioxidant methodology — in vivo and in vitro concepts. AOCS Press, Illinois, 23–38

    Google Scholar 

  69. Wiseman H, Halliwell B (1996) Damage to DNA by reactive oxygen and nitrogen species: Role in inflammatory disease and progression to cancer. Biochem J 313: 17–29

    PubMed  CAS  Google Scholar 

  70. Wong PS, Travis J (1980) Isolation and properties of alpha-1-proteinase inhibitor from human rheumatoid synovial fluid. Biochem Biophys Res Comm 96: 1449–1454

    Article  PubMed  CAS  Google Scholar 

  71. Chidwick K, Winyard PG, Zhang Z, Farrell AJ, Blake DR (1991) Inactivation of the elas-tase inhibitory activity of a1-antitrypsin in fresh samples of synovial fluid from patients with rheumatoid arthritis. Ann Rheum Dis 50: 915–916

    Article  PubMed  CAS  Google Scholar 

  72. Carp H, Miller F, Hoidal JR, Janoff A (1982) Potential mechanism of emphysema: alpha proteinase inhibitor recovered from lungs of cigarette smokers contains oxidised methionine and has decreased elastase inhibitory capacity. Proc Natl Acad Sci USA 79: 2041–2045

    Article  PubMed  CAS  Google Scholar 

  73. Matheson NR, Wong PS, Travis J (1979) Enzymatic inaction of human alpha-1-proteinase inhibitor by neutrophil myeloperoxidase. Biochem Biophys Res Comm 88: 402–409

    Article  PubMed  CAS  Google Scholar 

  74. Moreno JJ, Pryor WA (1994) Inactivation of a1-antiproteinase inhibitor by peroxynitrite. Chem Res Toxicol 5: 425–431

    Article  Google Scholar 

  75. Fu S, Gebicki S, Jessup W, Gebicki JM, Dean RT (1995) Biological fate of amino acid, peptide and protein hydroperoxides. Biochem J 311: 821–827

    PubMed  CAS  Google Scholar 

  76. Jiang ZY, Woodland ACS, Wolff SP (1992) Lipid hydroperoxide measurement by oxidation of Fe’ in the presence of xylenol orange: Comparison with the TBA assay and iodometric method. Lipids 26: 853–856

    Article  Google Scholar 

  77. Thomas SM, Jessup W, Gebicki JM, Dean RT (1989) A continuous-flow automated assay for iodometric estimation of hydroperoxides. Anal Biochem 176: 353–359

    Article  PubMed  CAS  Google Scholar 

  78. Gieseg SP, Simpson JA, Charlton TS, Duncan MW, Dean RT (1993) Protein bound, 3,4dihydroxyphenylalanine is a major reductant formed during hydroxyl radical damage to proteins. Biochem 32: 4780–4786

    Article  CAS  Google Scholar 

  79. Maskos Z, Rush JD, Koppenol WH (1992) The hydroxylation of tryptophan. Arch Biochem Biophys 296: 514–520

    Article  PubMed  CAS  Google Scholar 

  80. Griffiths HR, Lunec J, Blake DR (1992) Oxygen radical induced fluorescence in proteins; identification of the fluorescent tryptophan metabolite, N-formylkynurenine, as a biological index of radical damage. Amino Acids 3:183–194

    Article  CAS  Google Scholar 

  81. Uchida K, Kawakishi S (1994) Identification of oxidised histidine generated at the active site of Cu, Zn-superoxide dismutase exposed to H2O2. J Biol Chem 269: 2405–2410

    PubMed  CAS  Google Scholar 

  82. Kaur H, Halliwell B (1994) Aromatic hydroxylation of phenylalanine as an assay for hydroxyl radicals. Measurement of hydroxyl radical formation from ozone and in blood from premature babies using improved HPLC methodology. Anal Biochem 220: 11–15

    Article  PubMed  CAS  Google Scholar 

  83. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadman ER (1990) Determination of carbonyl content in oxidatively modified protein. Methods Enzymol 186: 464–487

    Article  PubMed  CAS  Google Scholar 

  84. Chapman ML, Rubin BR, Gracy RW (1989) Increased carbonyl content of proteins in synovial fluid from patients with rheumatoid arthritis. J Rheumatol 16: 15–18

    PubMed  CAS  Google Scholar 

  85. Levine RL, Williams JA, Stadman ER, Shacter (1994) Carbonyl assays for determinination of oxidatively modified proteins. Methods Enzymol 233: 346–357

    Article  PubMed  CAS  Google Scholar 

  86. Keller J, Halmes NC, Hinson JA, Pumford NR (1993) Immuno-chemical detection of oxidised proteins. Chem Res Toxicol 6: 430–433

    Article  PubMed  CAS  Google Scholar 

  87. Dizdaroglu M (1993) Chemistry of free radical damage to DNA and nucleoproteins. In: B Halliwell, OI Aruoma (eds): DNA and free radicals. Ellis Harwood, Chichester, UK, 19–39

    Google Scholar 

  88. Cadet J, Ravant JL, Buchko GW, Yeo HC, Ames BN (1994) Singlet oxygen DNA damage: chromatographic and mass spectrometric analysis of damage products. Methods Enzymol 234: 79–88

    Article  PubMed  CAS  Google Scholar 

  89. deRojas-Walker T, Tamir S, Ji H, Wishnock JS, Tennenbaum SR (1995) Nitric oxide induces oxidative damage in addition to deamination in macrophage DNA. Chem Res Toxicol 8: 473–477

    Article  PubMed  CAS  Google Scholar 

  90. Whiteman M, Jenner A, Halliwell B (1997) Hypochlorous acid-induced base modification in isolated calf thymus DNA. Chem Res Toxicol 10: 1240–1246

    Article  PubMed  CAS  Google Scholar 

  91. Halliwell B, Dizdaroglu M (1992) The measurement of oxidative damage to DNA by HPLC and GC/MS techniques. Free Rad Res Comm 16: 75–87

    Article  CAS  Google Scholar 

  92. Nackerdien Z, Olinski R, Dizdaroglu M (1992) DNA base damage in chromatin of g-irradiated cultured human cells. Free Rad Res Comm 16: 259–273

    Article  CAS  Google Scholar 

  93. Floyd RA, Watson JJ, Wong PK, Altmiller DH, Rickard RC (1986) Hydroxyl-free radical adduct of deoxyguanosine: sensitive detection and mechanism of formation. Free Rad Res Comm 1: 163–172

    Article  CAS  Google Scholar 

  94. Dizdaroglu M (1994) Chemical determination of oxidative DNA damage by gas chromatography-mass spectrometry. Methods Enzymol 234: 3–16

    Article  PubMed  CAS  Google Scholar 

  95. Jenner A, England TG, Aruoma OI, Halliwell B (1998) Measurement of oxidative DNA damage by gas chromatography/mass spectroscopy. The use of ethanethiol to prevent artefactual generation of oxidised DNA bases. Biochem J 331: 365–369

    PubMed  CAS  Google Scholar 

  96. Kaur H, Halliwell B (1996) Measurement of oxidised and methylated DNA bases by HPLC with electrochemical detection. Biochem J 318: 21–23

    PubMed  CAS  Google Scholar 

  97. McCord JM (1997) Free radicals and inflammation. Science 185: 529–531

    Article  Google Scholar 

  98. Halliwell B (1978) Superoxide-induced generation of hydroxyl radicals in the presence of iron salts. Its role in degradation of hyaluronic acid by a superoxide-generating system. FEBS Lett 96: 238–242

    Article  PubMed  CAS  Google Scholar 

  99. Li M, Rosenfield L, vilar RE and Cowman MK (1997) Degradation of hyaluronan by peroxynitrite. Arch Biochem Biophys 341: 245–250

    Article  PubMed  CAS  Google Scholar 

  100. Fairburn K, Grootveld M, Ward RJ, Abiuka C, Kus M, Williams RB, Winyard PG, Blake DR (1992) a-Tocopherol, lipids and lipoproteins in knee-joint synovial fluid and serum from patients with inflammatory joint disease. Clin Sci 83: 657–664

    PubMed  CAS  Google Scholar 

  101. Winyard PG, Tatzber F, Esterbauer H, Kus ML, Blake DR, Morris CJ (1993) Presence of foam cells containing oxidised low density lipoprotein in the synovial membrane from patients with rheumatoid arthritis. Ann Rheum Dis 52: 677–680

    Article  PubMed  CAS  Google Scholar 

  102. Yermilov V, Ribio J, Becci M, Friesen MD, Pignatelli B, Oshima H (1995) Formation of 8-nitroguanine by reaction of guanine with peroxynitrite in vivo. Carcinogenesis 16: 2045–2050

    Article  PubMed  CAS  Google Scholar 

  103. Kaur H, Halliwell B (1990) Action of biologically-relevant oxidising species upon uric acid. Identification of uric acid oxidation products. Chem Biol Interac 237: 314–321

    Google Scholar 

  104. Wasko P, Rotrosen D, Levine M (1989) Ascorbic acid transport and accumulation in human neutrophils. J Biol Chem 264: 18996–19002

    Google Scholar 

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Authors and Affiliations

  1. Department of Surgical Research, Northwick Park Institute of Medical Research, Watford Road, Harrow, Middlesex, HA1 3UJ, UK

    Harparkash Kaur

  2. Department of Biochemistry, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore

    Barry Halliwell

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  1. Harparkash Kaur
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  2. Barry Halliwell
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  1. St. Bartholomew’s and The Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, 25-29 Ashfield Street, London, E1 1AD, UK

    Paul G. Winyard

  2. School of Postgraduate Medicine, University of Bath, Claverton Down, Bath, BA2 7AY, UK

    David R. Blake

  3. Musculoskeletal Institute, University of Pittsburgh School of Medicine, 986 Scaife Hall, Pittsburgh, 15261, PA, USA

    Christopher H. Evans

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Kaur, H., Halliwell, B. (2000). Evolving measurements of radical products — how much do they tell us about inflammation?. In: Winyard, P.G., Blake, D.R., Evans, C.H. (eds) Free Radicals and Inflammation. Progress in Inflammation Research. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8482-2_16

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  • DOI: https://doi.org/10.1007/978-3-0348-8482-2_16

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