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Nanospray mass spectrometry in protein and peptide chemistry

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Proteomics in Functional Genomics

Part of the book series: EXS ((EXS,volume 88))

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Summary

The introduction of electrospray in the mid-1980s revolutionised biological mass spectrometry, in particular in the field of protein and peptide sequence analysis. Electrospray is a concentration-dependent, rather than a mass-dependent process, and maximum sensitivity is achieved at low flow rates with high-concentration, low-volume samples. This has lead to the development of nanoelectrospray, microelectrospray and related low flow-rate forms of electrospray which offer high sensitivity with low sample consumption. In this chapter the physical chemistry of low flow-rate electrospray is discussed, and a brief review of the types of low flow-rate electrospray interfaces is made. An indication of the performance obtainable on various instruments is given, along with some comments from the author’s own experience of these techniques.

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Author information

Authors and Affiliations

  1. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, SE-171 77, Sweden

    William J. Griffiths

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  1. William J. Griffiths
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Editor information

Editors and Affiliations

  1. Laboratoire de Chimie des Substances Naturelles URA C.N.R.S. No. 401, Muséum National d’Histoire Naturelle, 63, rue Buffon, F-75005, Paris, France

    P. Jollès

  2. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

    H. Jörnvall

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© 2000 Springer Basel AG

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Griffiths, W.J. (2000). Nanospray mass spectrometry in protein and peptide chemistry. In: Jollès, P., Jörnvall, H. (eds) Proteomics in Functional Genomics. EXS, vol 88. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8458-7_5

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  • DOI: https://doi.org/10.1007/978-3-0348-8458-7_5

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-9576-7

  • Online ISBN: 978-3-0348-8458-7

  • eBook Packages: Springer Book Archive

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