Summary
To investigate the possible interaction of mitochondrial aspartate aminotransferase (mAAT) with cytosolic components, a photoreactive probe was incorporated co-translationally into mAAT chains synthesized in a cell-free extract. Irradiation of the translation reaction results in the appearance of four crosslinked species containing radioactive mAAT with apparent molecular weights ranging from 70 to 200 kDa. These results suggest that newly synthesized mAAT is associated with as yet unknown factors from the cell extract. Fractionation of a fresh translation reaction by gel filtration chromatography confirms that indeed a large fraction of mAAT translation product is present as high molecular weight species.
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References
Mattingly, J. R., Jr., Rodriguez-Berrocal, F. J., Gordon, J., Iriarte, A. and Martinez-Carrion, M. (1987) Molecular Cloning andIn VivoExpression of a Precursor to Rat Mitochondrial Aspartate Aminotransferase.Biochem. Biophys. Res. Commun. 149:859–865.
Mattingly, J. R., Jr., Iriarte, A. and Martinez-Carrion, M. (1993) Structural Features Which Control Folding of Homologous Proteins in Cell-free Translation Systems.J. Biol. Chem. 268:26320–26327.
Crowley, K S., Reinhart, G. D. and Johnson, A. E. (1993) The Signal Sequence Moves through a Ribosomal Tunnel into a Noncytoplasmic Aqueous Environment at the ER Membrane Early in Translocation.Cell 73:1101–1115.
Krieg, U. C., Johnson, A. E. and Walter, P. (1987) Protein Translocation across the Endoplasmic Reticulum Membrane: Identification by Photocross-Linking of a 39-kD Integral Membrane Glycoprotein as Part of a Putative Translocation Tunnel.J. Cell Biol. 109:2033–2043.
Lain, B., Iriarte, A. and Martinez-Carrion, M. (1994) Dependence of the Folding and Import of the Precursor to Mitochondrial Aspartate Aminotransferase on the Nature of the Cell-free Translation System.J. Biol. Chem. 269:15588–15596.
Do, H., Falcone, D., Lin, J., Andrews, D. W. and Johnson, A. E. (1996) The Cotranslational Integration of Membrane Proteins into the Phospholipid Bilayer Is a Multistep Process.Cell 85:369–378.
Behra, R. and Christen, P. (1986) In Vitro Import into Mitochondria of the Precursor of Mitochondrial Aspartate Aminotransferase.J. Biol. Chem.261:257–263.
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© 2000 Springer Basel AG
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Lain, B., Tanase, C.A., Iriarte, A., Johnson, A.E., Martinez-Carrion, M. (2000). Association of Newly Synthesized Mitochondrial Aspartate Aminotransferase with Cytosolic Factors. In: Iriarte, A., Martinez-Carrion, M., Kagan, H.M. (eds) Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8397-9_40
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DOI: https://doi.org/10.1007/978-3-0348-8397-9_40
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-9549-1
Online ISBN: 978-3-0348-8397-9
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