Summary
Limited proteolysis and binding to GroEL were used to probe the conformations of the nascent mitochondrial aspartate aminotransferase synthesized in reticulocyte lysate and the denatured protein refoldingin vitro.Results suggest that the conformations of the partially folded proteins are similar. The GroEL—bound, refolding precursor can be imported into mitochondria in the presence of reticulocyte lysate but not in its absence. This suggests that a partially folded conformation alone is insufficient for import and that reticulocyte lysate factors are also needed.
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Mattingly, J.R., Torella, C., Yañez, A.J. (2000). A partially folded conformation is not the only requirement for import of mitochondrial aspartate aminotransferase. In: Iriarte, A., Martinez-Carrion, M., Kagan, H.M. (eds) Biochemistry and Molecular Biology of Vitamin B6 and PQQ-dependent Proteins. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8397-9_38
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DOI: https://doi.org/10.1007/978-3-0348-8397-9_38
Publisher Name: Birkhäuser, Basel
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Online ISBN: 978-3-0348-8397-9
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