Abstract
This chapter’s primary emphasis is on providing readers with a practical understanding of microsatellites, so that the technical aspects of obtaining them, using them for genotyping, and analyzing data become straightforward. In order to do so, we first provide some general background on microsatellites, which leads us into more practical protocols and suggestions. The majority of the techniques are centered around fluorescent dye labeling and automated DNA sequencer technology, since many laboratories are moving in this direction. However, we do provide some brief tips and discussion on the use of radioisotopes because they are still used and provide high-quality data. Towards the end of this chapter, we review some specific concerns and troubleshooting ideas when calling microsatellite alleles (or lack thereof). For problems that occur at the early stage of PCR amplification, we offer a troubleshooting flow chart for thermocycler reactions. Although we refer to other reports for details on technical aspects of microsatellites, it is our hope that this chapter
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Birnbaum, K.D., Rosenbaum, H.C. (2002). A Practical Guide for Microsatellite Analysis. In: DeSalle, R., Giribet, G., Wheeler, W. (eds) Techniques in Molecular Systematics and Evolution. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8125-8_16
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DOI: https://doi.org/10.1007/978-3-0348-8125-8_16
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-7643-6257-7
Online ISBN: 978-3-0348-8125-8
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