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Purification, characterization and mechanism of action of scatter factor from human placenta

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Cell Motility Factors

Part of the book series: Experientia Supplementum ((EXS,volume 59))

Summary

Scatter factor (SF) causes contiguous sheets of epithelium to spread and cells to separate from each other. SF also increases the velocity, area, and reduces the circularity of individual cells. These changes are mediated in part by alterations in protein synthesis, protein phosphorylation, cytoskeletal reorganization, and cell surface components. SF has been purified from the conditioned medium of ras transformed 3T3 cells and human placenta. Sequence information suggests that SF from 3T3 cells is closely related to hepatocyte growth factor. SF is a glycoprotein, but glycosylation is not necessary for its activity. Glycosylation of target cell proteins, however, is required for SF action.

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Author information

Authors and Affiliations

  1. Department of Radiation Oncology, Long Island Jewish Medical Center, The Long Island Campus of Albert Einstein College of Medicine, 11042, New Hyde Park, NY, USA

    Madhu M. Bhargava, Yuan Li, Ansamma Joseph, Maryanne Pendergast, Regina Hofmann, Eliot M. Rosen & Itzhak D. Goldberg

  2. Department of Radiation Therapy, Yale University Medical School, 06510, New Haven, CT, USA

    Eliot M. Rosen

Authors
  1. Madhu M. Bhargava
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  2. Yuan Li
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  3. Ansamma Joseph
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  4. Maryanne Pendergast
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  5. Regina Hofmann
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  6. Eliot M. Rosen
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  7. Itzhak D. Goldberg
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Editor information

Editors and Affiliations

  1. Department of Radiation Oncology, Long Island Jewish Medical Center, 270-05 76th Avenue, 11042, New Hyde Park, NY, USA

    I. D. Goldberg M.D.

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© 1991 Birkhäuser Verlag Basel/Switzerland

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Bhargava, M.M. et al. (1991). Purification, characterization and mechanism of action of scatter factor from human placenta. In: Goldberg, I.D. (eds) Cell Motility Factors. Experientia Supplementum, vol 59. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7494-6_5

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  • DOI: https://doi.org/10.1007/978-3-0348-7494-6_5

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7496-0

  • Online ISBN: 978-3-0348-7494-6

  • eBook Packages: Springer Book Archive

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