DNA damage in mammalian cell lines with different antioxidant levels and DNA repair capacities
A wide range of DNA damage is known to be caused by reactive oxygen species (ROS). Defence against the effects of such damage include damage prevention (e.g. antioxidant activity) and the removal of damaged moieties from DNA (DNA repair). Radiation (X-ray) sensitive murine lymphoma (LY) cells were seen to be more susceptible to ROS-induced damage than were radiation resistant cells. This difference was unlikely to be due to the marginally decreased DNA excision repair capacity of the sensitive cells. Radiation sensitive cells did, however, have lower endogenous antioxidant enzyme levels. Thus, the importance of assessing all levels of a cell’s response to ROS, in determining the major factors leading to increased mutagen sensitivity, is emphasised.
KeywordsReactive Oxygen Species Excision Repair L5178Y Cell Murine Lymphoma Cell L5178Y Mouse Lymphoma
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- Aebi, H. (1983) Catalase in vitro, in: Methods of Enzymatic Analysis. H. U. Bergmeyer, ed. 3rd Ed. Veriag Chemie, Weinheim, Germany.Google Scholar
- Halliwell, B., and Gutteridge, J. M. C. (1989) Free Radicals in Biology and Medicine. (2nd ed.) Clarendon Press, Oxford.Google Scholar
- Jones, D. G., and Suttle, N. F. (1981) The effect of copper deficiency on leukocyte function in sheep and cattle. Res. in Vet. Sci. 31: 151–156.Google Scholar
- Richardson, S.-A. M., Hannigan, B. M., and McKenna, P. G. (1990a) DNA repair of oxidative damage in the cell line L517Y(S). Int. J. Rad. Biol. 57: 1257.Google Scholar
- Richardson, S.-A. M., Hannigan, B. M., and McKenna, P. G. (1990b) Assessment of unscheduled DNA synthesis (UDS) in murine lymphoma cells exposed to enzymatically generated oxidants, Br. J. Cancer 62: 530.Google Scholar
- Richardson, S.-A. M., Hannigan, B. M., and McKenna, P. G. (1991) DNA repair induced by reactive oxygen species (ROS) in the Friend erythroleukaemia cell line 707 Ir. J. Med. Sci. 160: 149.Google Scholar
- Strain, J. J., Hannigan, B. M., and McKenna, P. G. (1991) The pathophysiology of oxidant damage. J. Biomed. Sci. 2: 19–24.Google Scholar