Free radicals, lipid peroxidation, SOD activity, neurotransmitters and choline acetyltransferase activity in the aged rat brain
The mechanism of aging is suggested to be related to oxygen free radicals. Free radicals, lipid peroxidation and SOD activity have been reported to be increased in the aged brain. A Japanese herbal medicine, Sho-saiko-to-go-keishi-ka-shakuyaku-to (TJ-960), which has scavenging activities against hydroxyl radicals, superoxide, l,l-diphenyl-2-picrylhydrazyl radicals, carbon-centered radicals and alpha-tocopheroxyl radicals, decreased carbon-centered radicals and thiobarbituric acid reactive substances (TBARS) levels in the aged rat brain after a 3-week oral administration of 5% TJ-960 solution. TJ-960 elevated superoxide dismutase (SOD) activity in the cytosol fraction of the hippocampus and hypothalamus of aged rats. It decreased norepinephrine and 5-hydroxytryptamine (5-HT) levels in the hypothalamus and increased the 5-HT level in the cerebellum. TJ-960 treatment increased choline acetyltransferase activity in aged rats. As herbal medicines do not generally have harmful side effects, antioxidant TJ-960 appears to be a suitable prophylactic agent against some neuronal symptoms of aging.
KeywordsDown Syndrome Human Cerebrospinal Fluid Japanese Herbal Medicine Spin Trap Method Choline Acetyltrans
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- De Quiroga, G. B., Perez-Campo, R., and Lopezz Torres, M. (1990) Anti-oxidant defences and peroxidation in liver and brain of aged rats. Biochem. J. 272: 247–250.Google Scholar
- Hiramatsu, M., Edamatsu, R., Kabuto, H., and Mori, A. (1988a) Effect of Sho-saiko-to-go- keishi-ka-shakuyaku-to (TJ-960) on monoamines, amino acids, lipid peroxides, and superoxide dismutase in brains of aged rats in: Recent Advances in the Pharmacology of KAMPO (Japanese herbal medicines) (E. Hosoya and Y. Yamamura, Eds). Excerpta Medica, pp. 128–135.Google Scholar
- Hiramatsu, M., Edamatsu, R., Kohno, M., and Mori, A. (1988b) Scavenging of free radicals by Sho-saiko-to-go-keishi-ka-shalcuyaku-to (TJ-960) in: Recent Advances in the Pharmacology of KAMPO (Japanese herbal medicines) (E. Hosoya and Y. Yamamura, Eds). Excerpta Medica, pp. 120–127.Google Scholar
- Hiramatsu, M., Kabuto, H., and Mori, A. (1986) Effects of shosaiko-to-go-keishi-ka- shakuyaku-to (TJ-960) on brain catecholamine level of aged rats. IRCS Med. Sci. 14: 189–190.Google Scholar
- Hiramatsu, M., Kabuto, H., and Mori, A. (1988c) Effects of Sho-saiko-to-go-keishi-ka- shakuyaku-to (TJ-960) on convulsions and brain 5-hydroxytryptamine in El mice, in: Recent Advances in the Pharmacology of KAMPO (Japanese herbal medicines) E. Hosoya and Y. Yamamura, Eds. Excerpta Medica, pp. 69–73.Google Scholar
- Hiramatsu, M., and Kohno, M. (1987) Determination of superoxide activity by electron spin resonance spectrometry using the spin trap method. JEOL News 23A: 7.Google Scholar
- Hiramatsu, M., Velasco, R. D., and Packer, L. (1990) Vitamin E radical reaction with antioxidants in rat liver membranes. Free Rad. Biol. Med. 9: 459–464.Google Scholar