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The metabolism of 4-hydroxynonenal, a lipid peroxidation product, is dependent on tumor age in Ehrlich mouse ascites cells

  • Werner G. Siems
  • Tilman Grune
  • Beatrix Beierl
  • Helmward Zollner
  • Hermann Esterbauer
Part of the EXS book series (EXS, volume 62)

Summary

4-Hydroxynonenal is a major product formed by lipid peroxidation from omega 6-polyunsaturated fatty acids as linoleic acid and arachidonic acid. This aldehyde is cytotoxic at high concentrations (in the range of 100 μM), disturbs cell prohferation at low concentrations and exhibits genotoxic effects. Furthermore, in the submicromolar range 4-hydroxynonenal is chemotactic and stimulates phospholipase C. 4-Hydroxynonenal is rapidly metabolized in eucaryotic cells. Here the metabolism of 4-hydroxynonenal was studied in suspensions of Ehrlich mouse ascites cells at different periods of the tumor age. The Ehrlich ascites tumor is a convenient biological model for the investigation of tumor cells in different age and proliferation phases of the tumor. The main products of 4-hydroxynonenal which were identified in the Ehrlich ascites cells were glutathione-HNE-conjugate, hydroxynonenoic acid and 1,4-dihydroxynonene. The formation of glutathione conjugates following the addition of 4-hydroxynonenal was higher in cells of the early phase in comparison with cells of the late phase of tumor growth. That was in accordance with the increased consumption of the reduced form of glutathione during 4-hydroxynonenal utilization. The degradation of 4-hydroxynonenal and other aldehydic products of lipid peroxidation is postulated to be an important part of the intracellular antioxidative defense system.

Keywords

Lipid Peroxidation Lipid Peroxidation Product Ehrlich Ascites Tumor Cell Pyruvate Ratio Ehrlich Ascites Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

EATC

Ehrlich ascites tumor cells

GSH

glutathione (reduced form)

HNE

4-hydroxynonenal

DHN

1,4-dihydroxynonene

HNA

4-hydroxy-2-nonenoic acid

PUFA

polyunsaturated fatty acids.

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Copyright information

© Birkhäuser Verlag Basel/Switzerland 1992

Authors and Affiliations

  • Werner G. Siems
    • 1
  • Tilman Grune
    • 1
  • Beatrix Beierl
    • 2
  • Helmward Zollner
    • 2
  • Hermann Esterbauer
    • 1
  1. 1.Institute of Biochemistry, Medical Faculty (Charité)Humboldt University of BerlinBerlinFederal Republic of Germany
  2. 2.Institute of BiochemistryUniversity of GrazGrazAustria

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