Summary
α-Tocopherol significantly perturbs the phase transition of fully saturated phospholipids and its effect is considerably bigger than that of α-tocopheryl acetate as observed by differential scanning calorimetry and Fourier-transform infrared spectroscopy. This observation remarks the importance of its phenolic hydroxy1 group in determining the interaction of α-tocopherol with phospholipids. However, this hydroxyl group is not hydrogen bonded to neither the carbonyl group nor the phosphate group of phosphatidylcholine, although it seems able of forming a hydrogen bonding with the carbonyl group of phosphatidylethanolamine. By studying the intrinsic fluorescence of α-tocopherol, it was found that its chromanol group is located near the water-lipid interface, although it is not accesible from the water phase. On the other hand, α-tocopherol was found to partition with preference for the most fluid domains in the membrane, as seen by differential scanning calorimetry.
The dynamics of α-tocopherol in membranes was examined through the quenching of its intrinsic fluorescence was examined found to have a diffusion coefficient similar to that of phospholipids.
α-tocopherol was also found to promote the Hii phase of phosphatidylethanolamine, having a stronger effect than α-tocopherol acetate.
Finally, α-tocopherol was shown to have a stabilizing effect on liposomes, when they were stored for periods of time longer than one year.
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© 1991 Birkhäuser Verlag Basel/Switzerland
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Gómez-Fernández, J.C., Aranda, F.J., Villalain, J. (1991). Location and Dynamics of α-Tocopherol in Membranes. In: Gomez-Fernandez, J.C., Chapman, D., Packer, L. (eds) Progress in Membrane Biotechnology. ALS Advances in Life Sciences. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7454-0_8
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DOI: https://doi.org/10.1007/978-3-0348-7454-0_8
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