Abstract
Correct targeting of nuclear-encoded mitochondrial proteins is a complex process which has been intensively studied over the past fifteen years. Essential features have been identified in the structure of imported polypeptides which recognize specific receptors at the mitochondrial surface. Moreover, several cytosolic factors have been shown to be involved in the translocation and import of cytoplasmically synthesised mitochondrial proteins (Glover and Lindsay, 1992).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Baker, K. P., and Schatz, G. (1991) Mitochondrial proteins essential for viability mediate protein import into yest mitochondria. Nature (London) 349: 205–208.
Barile, M., Giannattasio, S., Marra, E., Passarella, S., Pucci, P., Sarnia, G. and Quagliariello, E. (1990) Certain N-terminal peptides inhibit uptake of mature aspartate aminotransferase by isolated mitochondria. Biochem. Biophys. Res. Commun. 170: 609–615.
Doonan, S., Marra, E., Passarella, S., Saccone, C. and Quagliariello, E. (1984) Transport of proteins into mitochondria. Int. Rev. Cytol. 91: 141–186.
Giannattasio, S., Marra, E., Abruzzese, M.F., Greco, M. and Quagliariello, E. (1991) The in vitro-synthesised precursor and mature mitochondrial aspartate aminotransferase share the same import pathway in isolated mitochondria. Arch. Biochem. Biophys. 290: 528–534.
Giannattasio, S., Marra, E., Vacca, R.A., Iannace, G. and Quagliariello, E. (1992) Import of mutant forms of mitochondrial aspartate aminotransferase into isolated mitochondria. Arch. Biochem. Biophys. 298: 532–538.
Giannattasio, S., Azzariti, A., Marra, E. and Quagliariello, E. (1994) The N-terminal region of mature aspartate aminotransferase can direct cytosolic dihydrofolate reductase into mitochondria in vitro. Biochem. Biophys. Res. Commun. (in press).
Glover, L.A. and Lindsay, J.G. (1992) Targeting proteins to mitochondria: a corrent overview. Biochem. J. 284: 609–620.
Graf-Hausner, U., Wilson, K.J. and Christen, P. (1983) The covalent structure of mitochondria] aspartate aminotrnsferase from chicken. J. Biol. Chem. 258: 8813–8826.
Hartmann, C. M., Gehring, H., and Christen, P. (1993) The mature form of imported mitochondrial proteins undergoes conformational changes upon binding to isolated mitochondria. Eur. J. Biochem. 218: 905–910.
Hurt, E. C. and van Loon, A. P. G. M. (1986) How proteins find mitochondria and intramitochondria compartments. Trends Biochem. Sci. 11: 204–207.
Marra, E, Passarella, S., Doonan, S., Saccone, C. and Quagliariello, E. (1979) The effect of sulphydryl group reagents on the permeation of mitochondrial aspartate aminotransferase into mitochondria in vitro. Arch. Biochem. Biophys. 195: 269–279.
Marra, E., Passarella, S., Doonan, S., Quagliariello, E. and Saccone, C. (1980) Protease resistance of aspartate aminotransferase imported in mitochondria. FEBS Lett. 122: 33–36.
Marra, E., Passarella, S., Casamassima, E., Perlino, E., Doonan, S. and Quagliarielli, E. (1985) Kinetic studies of the uptake of asparate aminotransferase and malate dehydrogenase into mitochondria in vitro. Biochem. J. 228: 493–503.
Mattingly, J. R., Jr., Iriarte, A., and Martinez-Carrion, M. (1993) Structural features which control folding of homologous proteins in cell-free translation systems. The effect of a mitochondrial-targeting presequence on aspartate aminotransferase isozymes. J. Biol. Chem. 268: 26320–26327.
Neupert, W., Hart!, F.-U., Craig, E. A., and Pfanner, N. (1990) How do polypeptides cross the mitochondrial membranes? Cell 63: 447–450.
O’Donovan, K.M.C., Doonan, S., Marra, E., Passarella, S. and Quagliariello, E. (1985) Removal of an N-terminal peptide from mitochondrial aspartate aminotransferase abolishes its interactions with mitochondria in vitro. Biochem. J. 228: 609–614.
Thompson, L. M., and McAllister-Henn, L. (1989) Disposable presequence for cellular localization and function of mitochondrial malate dehydrogenase from Saccharomyces cerevisiae. J. Biol. Chem. 264: 12091–12096.
Volland, C., and Urban-Grimal, D. (1988) The presequence of yeast 5-aminolevulinate synthase is not required for targeting to mitochondria. J. Biol. Chem. 263: 8294–8299.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Birkhäuser Verlag Basel/Switzerland
About this paper
Cite this paper
Giannattasio, S., Azzariti, A., Vacca, R.A., Lattanzio, P., Merafina, R.S., Marra, E. (1994). Mitochondrial protein import: role of certain domains of mitochondrial aspartate aminotransferase. In: Marino, G., Sannia, G., Bossa, F. (eds) Biochemistry of Vitamin B6 and PQQ. Advances in Life Sciences. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7393-2_13
Download citation
DOI: https://doi.org/10.1007/978-3-0348-7393-2_13
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-7395-6
Online ISBN: 978-3-0348-7393-2
eBook Packages: Springer Book Archive