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A Laboratory Guide to Glycoconjugate Analysis pp 141–160Cite as

Carbohydrate Analysis with Capillary Electrophoresis

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Part of the book series: BioMethods ((BIOMETHODS))

Abstract

Capillary electrophoresis (CE) is an automated, high-resolution, miniaturized separation technique. CE instrumentation is similar to high-pressure liquid chromatography (HPLC) and allows unattended operation with on-column detection and computer-aided data processing. Typically, several hundred thousand theoretical plates per column can be achieved in run times of less than 20 min. The separation is accomplished in fused-silica capillaries of 50–100 µm inner diameter (ID) and 20–50 cm in length. The application of a high electrical field in the order of several hundred V/cm induces a differential migration of charged solutes, making CE an attractive separation tool, especially for biological samples.

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Authors
  1. Antje Klockow-Beck
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  2. Aran Paulus
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Editor information

Editors and Affiliations

  1. Division of Transfusion Medicine, University of Cambridge East Anglian Blood Centre, Long Road, CB2 2PT, Cambridge, UK

    Peter Jackson

  2. Cancer Research Campaign Department of Medical Oncology, University of Manchester Christie CRC Research Centre, Wilmslow Road, M20 4BX, Manchester, UK

    John T. Gallagher

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© 1997 Birkhäuser Verlag Basel

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Klockow-Beck, A., Paulus, A. (1997). Carbohydrate Analysis with Capillary Electrophoresis. In: Jackson, P., Gallagher, J.T. (eds) A Laboratory Guide to Glycoconjugate Analysis. BioMethods. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7388-8_8

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  • DOI: https://doi.org/10.1007/978-3-0348-7388-8_8

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7390-1

  • Online ISBN: 978-3-0348-7388-8

  • eBook Packages: Springer Book Archive

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