Summary
Mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive DNA elements known as VNTRs. Since a high GC-content is characteristic of most of these elements one might expect them to be clustered at least partially in CpG-islands. To address this question we have constructed a porcine liver DNA NotI-linkage library in pUC18 using isolated HTF-islands. HpaII tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and analysed by sequencing. As expected from the source of DNA the clones were rich in CpG-, C- and G-content. Several clones exhibiting a repetitive (GGC)n, motif and sequences originally present in human VNTR-markers were shown to detect DNA fingerprints in different species (Homo sapiens, Sus scrofa domestica,Gallus domesticus, Glycine max, Saccharomyces cerevisiae). A subset of clones was used for hybridization experiments with porcine DNA and were shown to detect single-copy linkage fragments.
Keywords
- Globin Gene
- Volume Molar Excess
- Gallus Domesticus
- Human Major Histocompatibility Complex Class
- Linkage Fragment
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Smith, J. A., Seidman, J. G., and Struhl, K. (eds.) (1987) Current protocols in molecular biology. Wiley & Sons, New York.
Bird, A. P., Taggert, M., Frommer, M., Miller, O. J., and Mcleod, D., (1985) A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA. Cell 40: 91–99.
Burke, T., Davies, N. B., Bruford, M. W., and Hatchwell, B. J. (1989) Parental care and mating behaviour of polyandrous dunnocks Prunella modularis related to paternity by DNA fingerprinting. Nature 338: 249–251.
Church, G. M., and Gilbert, W. (1984) Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991–1995.
Coles, L. S., Robins, A. J., Madley, L. K., and Wells, J. R. E. (1987) Characterization of the chicken histone HI gene complement. J. Biol. Chem. 262: 9656–9663.
Dallas, J. F. (1988) Detection of DNA “fingerprints” of cultivated rice by hybridization with a human minisatellite DNA probe. Proc. Natl. Acad. Sci. USA 85: 6831–6835.
Feinberg, A. P., and Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132: 6–13.
Gardiner-Garden, M., and Frommer, M. (1987) CpG islands in vertebrate genomes. J. Mol.Biol. 196: 261–282.
Goodbourn, S. E. Y., Higgs, D. R., Clegg, J. B., and Weatherall, D. J. (1983) Molecular basis of length polymorphism in the human -globin gene complex. Proc. Natl. Acad. Sci. USA 80: 5022–5026.
Gyllensten, U. B., Jakobsson, S., Temrin, H., and Wilson, A. C. (1989) Nucleotide sequence and organization of bird minisatellites. Nucl. Acids Res. 17: 2203–2214.
Haley, C. S., Archibald, A., Andersson, L., Bosma, A. A., Davies, W., Fredholm, M., Geldermann, H., Groenen, M., Gustaysson, I., Ollivier, L., Tucker, E. M., and Van de Weghe, A. (1990) The pig gene mapping project—PiGMaP. Proceedings of the 4th World Congress on Genetics Applied to Livestock Production XIII: 67–70.
Hanahan, D. (1987) Techniques for transformation of E. coli. In: DNA cloning Vol. I, A practical approach (D. M. Glover, ed.), IRL Press.
Huxley, C., and Fried, M. (1990) The mouse surfeit locus contains a cluster of six genes associated with four CpG-rich islands in 32 kilobases of genomic DNA. Mol. Cell. Biol. 10: 605–614.
Ito, T., and Sakaki, Y. (1989) A novel procedure for selective cloning of NotI-linking fragments from mammalian genomes. Nucl. Acids Res. 16: 9177–9184.
Jeffreys, A. J., Brookfield, J. F. Y., and Semenoff, R. (1985a) Positive identification of an immigration test-case using human DNA fingerprints. Nature 317: 818–819.
Jeffreys, A. J., Wilson, V., and Thein, L. (1985b) Individual-specific “fingerprints” of human DNA. Nature 316: 76–79.
Jeffreys, A. J., Wilson, V., and Thein, S. L. (1985c) Hypervariable “minisatellite” regions in human DNA. Nature 314: 67–73.
Jorcano, J. L., Rieger, M., Franz, J. K., Schiller, D. L., Moll, R., and Franke, W. W. (1984) Identification of two types of keratin polypeptides within the acidic cytokeratin subfamily I. J. Mol. Biol. 179: 257–281.
Lindsay, S., and Bird, A. P. (1987) Use of restriction enzymes to detect potential gene sequences in mammalian DNA. Nature 327: 336–338.
Nakamura, Y., Leppert, M., O’Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlian, E., and White, R. (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616–1622.
Roberts, L. (1990) An animal genome project? Science 248: 550–552.
Sanger, F., Micklen, S., and Coulsen, A. R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463–5467.
Sargent, C. A., Dunham, I., and Campbell, R. D. (1989) Identification of multiple HTF-island associated genes in the human major histocompatibility complex class III region. EMBO J. 8: 2305–2312.
Sullivan, K. F., Havercroft, J. C., Machün, P. S., and Cleveland, D. W. (1986) Sequence and expression of the chicken ß5- and ß4-tubulin genes define a pair of divergent ß-tubulins with complementary patterns of expression. Mol. Cell. Biol. 6: 4409–4418.
Tautz, D. (1989) Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl. Acids Res. 17: 6463–6471.
Vieria, J., and Messing, J. (1982) The pUC-plasmids, a M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19: 259–268.
White, R., Leppert, M., Bishop, D. T., Barker, D., Berkowitz, J., Brown, C., Callahan, P., Holm, T., and Jerominski, L. (1985) Construction of linkage maps with DNA markers for human chromosomes. Nature 313: 101–105.
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© 1991 Birkhäuser Verlag Basel/Switzerland
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Brenig, B., Brem, G. (1991). Human VNTR Sequences in Porcine HTF-Islands. In: Burke, T., Dolf, G., Jeffreys, A.J., Wolff, R. (eds) DNA Fingerprinting: Approaches and Applications. Experientia Supplementum, vol 58. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7312-3_3
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DOI: https://doi.org/10.1007/978-3-0348-7312-3_3
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