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Stimulation of 5-HT3 Receptors Inhibits Release of Endogenous Noradrenaline from Hypothalamus

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Serotonin: Molecular Biology, Receptors and Functional Effects

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Summary

Exposure to 20 mM KCl elicited a Ca++-dependent release of endogenous NA (noradrenaline) from superfused rat hypothalamic slices. Two consecutive exposures, S1 and S2, respectively, produced NA release of similar magnitude (S2/S1 = 1.01 ± 0.07, n = 5). 5-HT (5-hydroxytryptamine), 10µM, inhibited KC1-evoked NA release by 50%, in the presence but not in the absence of the 5-HT2/5-HT1C receptor antagonist ritanserin. 2-ME-5-HT (2-methy-l5-hydroxytryptamine), a selective 5-HT3 receptor agonist, but not α-ME-5-HT (α-methyl-5-hydroxytryptamine), a 5-HT1-like and 5-HT2 receptor agonist, mimicked the 5-HT response in the presence and in the absence of ritanserin. ICS 205–930 ((3α-tropanyl)1H-indole-carboxylic acid ester), 1 nM, and s(-)zacopride, 3 nM, highly selective 5-HT3 receptor antagonists, inhibited the effects of both agonists. These observations provide direct evidence for a 5-HT-mediated modulation of endogeneous NA release from rat hypothalamus.

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Authors and Affiliations

  1. Department of Pharmacology, Mount Sinai Medical School of the City University of New York, New York, 10029, NY, USA

    P. Blandina, J. Goldfarb & J. P. Green

  2. Dipartimento di Farmacologia Preclinica e Clinica, Universitá degli Studi di Firenze, 50134, Firenze, Italia

    P. Blandina

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  2. J. Goldfarb
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  1. Preclinical Research, Sandoz Pharma Ltd., P.O. Box 6, CH-4002, Basel, Switzerland

    John R. Fozard

  2. Institute of Pharmacology, Erasmus University Rotterdam, P.O. Box 1738, NL-3000 DR, Rotterdam, The Netherlands

    Pramod R. Saxena

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© 1991 Birkhäuser Verlag Basel/Switzerland

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Blandina, P., Goldfarb, J., Green, J.P. (1991). Stimulation of 5-HT3 Receptors Inhibits Release of Endogenous Noradrenaline from Hypothalamus. In: Fozard, J.R., Saxena, P.R. (eds) Serotonin: Molecular Biology, Receptors and Functional Effects. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7259-1_30

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  • DOI: https://doi.org/10.1007/978-3-0348-7259-1_30

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7261-4

  • Online ISBN: 978-3-0348-7259-1

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