Abstract
Towards the end of the sixties, a new type of scanning electron microscope was developed by Albert Crewe and his graduate students in Chicago. Combining state-of-the-art electron optics with a field-emission gun, it was possible to demonstrate that a scanning microscope can achieve near atomic resolution. In addition, as outlined in a remarkable article by Crewe in 1970 (2), the scanning transmission electron microscope (STEM) should allow several novel imaging modes ideally suited for visualizing fragile biomacromolecules (for STEM imaging modes see Fig. 1). These revolutionary ideas and the first stunning dark field micrographs of unstained DNA molecules were presented in 1970 at the International Congress of Electron Microscopy in Grenoble. The enthusiasm induced by these results among the biologically oriented electron microscopists may be compared to the recent excitement fostered by the scanning tunneling microscope (STM) invented by Heinrich Rohrer and Gerd Binnig in Rüschlikon (1).
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© 1990 Birkhäuser Verlag Basel
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Engel, A. (1990). The Swiss Stem Project. In: Günter, J.R. (eds) History of Electron Microscopy in Switzerland. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7203-4_2
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DOI: https://doi.org/10.1007/978-3-0348-7203-4_2
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