Abstract
A heterogeneous, double-antibody, fluorometric enzyme-linked immunosorbent assay (ELISA) is described for the detection and quantitation of metallothionein (MT). The protocol uses the same immunological reagents as the radioimmunoassay (RIA) developed previously in this Laboratory; fluorescence replaces radioactivity for detection of the reference antigen in the specific binding reaction. Present results indicate that the developed ELISA has approximately the same range of capability in detecting and quantitating MT as is characteristic of the RIA. The ELISA has the advantage that the time required to perform a typical assay is significantly less than that required for the RIA.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Vander Mallie, R.J. and Garvey, J.S. (1978) Immunochem. 15, 857–868.
Vander Mallie, R.J. and Garvey, J.S. (1979) J. Biol. Chem. 254, 8416–8421.
Garvey, J.S., Vander Mallie, R.J. and Chang, C.C. (1982) Methods Enzymol. 84, 121–138.
Msrgoshes, M. and Vallee, B.L. (1957) J. Am. Chem. Soc. 79, 4813.
Winge, D.R. and Garvey, J.S. (1983) Proc. Nat. Acad. Sci. (USA) 80, 2472–2476.
Garvey, J.S. (1984) Env. Health Perspect. 54, 117–127.
Chang, C.C., Lauwerys, R., Bernard, A., Roels, H., Buchet, J.P. and Garvey, J.S. (1980) Environ. Res. 23, 422–428.
Roels, H., Lauwerys, R., Buchet, J.P., Bernard, A., Garvey, J.S. and Linton, H.J. (1983) Int. Arch. Occup. Environ. Health 52, 159–166.
Nordberg, G.F., Garvey, J.S. and Chang, C.C. (1982) Environ. Res. 28, 179–182.
Falck, F.Y., Fine, L.J., Smith, R.G., Garvey, J.S., Schork, A.M., England, B.G., McClatchey, K.D. and Linton, H.J. (1983) Brit. J. Ind. Med. 40, 305–313.
Garvey, J.S. and Chang, C.C. (1981) Science 214, 805–807.
Hart, B.A. and Garvey, J.S. (1985) Environ. Res. (in press).
Zimmerman, A.W., Garvey, J.S., Banta, J.V. and Horak, E. (1985) Pediatr. Neurol. 1, 23–27.
Garvey, J.S. (1982) In: “Nephrotoxic Mechanisms of Drugs and Environmental Toxins”, G.A. Porter ed., Plenum, New York, pp. 437–449.
Winge, D.R., Geller, B.L. and Garvey, J.S. (1981) Arch. Biochem. Biophys. 208, 160–166.
Zelazowski, A.J., Garvey, J.S. and Hoeschele, J.D. (1984) Arch. Biochem. Biophys. 229, 246–252.
Laib, J.E., Shaw, C.F. III, Petering, D.H., Eidsness, M.K., Elder, R.C. and Garvey, J.S. (1985) Biochem. 24, 1977–1986.
Linton, H.J. (1983) M.S. Thesis, Graduate School, Syracuse University, Syracuse, New York.
Engvall, E. (1980) Methods Enzymol. 70, 419–439.
Voller, A., Bidwell, D.E. and Bartlett, A. (1976). In: “Immunoenzymatic Assay Techniques”, R. Malvano ed., Martinus Nijhoff, The Hague, pp. 104–115.
Engvall, E. and Perlmann, P. (1972) J. Immunol. 109, 129–135.
Pesce, A.J., Krieger, N.J. and Michael, J.G. ( 1983 In: “Immunoenzymatic Techniques”, S. Avrameas, P. Druet, R. Masseyeff and G. Feldmann eds., Elsevier, Amsterdam, pp. 127–138.
Rodbard, D., Feldman, Y., Jaffe, M.L. and Miles, L.E.M. (1978) Immunochem. 15, 77–82.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Basel AG
About this chapter
Cite this chapter
Garvey, J.S., Thomas, D.G., Linton, H.J. (1987). Enzyme-Linked Immunosorbent Assay (ELISA) for Metallothionein. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_31
Download citation
DOI: https://doi.org/10.1007/978-3-0348-6784-9_31
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-6786-3
Online ISBN: 978-3-0348-6784-9
eBook Packages: Springer Book Archive