Abstract
Here, results are presented of studies on procedures, by which new properties residing on genes foreign to the host could be introduced into plants. The approach was based on the use of a naturally occurring system of genetic manipulation of plants as it is performed by the soil-bacterium Agrobacterium tumefaciens. Infection of wounded plants with this bacterium results in an unlimited proliferation of plant cells, which form a plant tumor, that is called crown gall. The cause of this disease is the expression in plant cells of a piece of bacterial DNA derived from an extrachromosomal, circular DNA or plasmid of the bacterium1. The transferred part of it is called T-DNA, While this segment is named T-region as part of the Ti-plasmid. By inserting new genes in this region of the Ti-plasmid it should be possible to transfer these genes into plant cells.
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Krens, F.A., Wullems, G.J., Schilperoort, R.A. (1983). A Reproducible Method for the Introduction of Nucleic Acids in Tobacco Protoplasts. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_65
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DOI: https://doi.org/10.1007/978-3-0348-6556-2_65
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