Abstract
Regeneration of plants through protoplast provides a unique potential for modifying the genetic complement of agriculturally important crops. This procedure begins with protoplast isolation, followed by wall regeneration, heterokaryon formation and ultimately leading to hybrid cells, 1,2,3. These cells undergo morphogenesis and produce plants. This paper describes, methods of isolation of protoplasts from Moss protonema. These protoplasts are capable of cell wall regeneration, sustained cell division, protonema bud formation ultimately leading to gametophytes and capsule formation.
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References
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© 1983 Springer Basel AG
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Batra, A., Abel, W.O. (1983). Protoplast Isolation, Culture and Plant Formation of Psycomitrella. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_20
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DOI: https://doi.org/10.1007/978-3-0348-6556-2_20
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-6557-9
Online ISBN: 978-3-0348-6556-2
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