Skip to main content
SpringerLink
Log in

Developmental Potential of Mega and Normal Protoplasts in Populus

  • Chapter
Protoplasts 1983

Part of the book series: EXS 45: Experientia Supplementum ((EXS,volume 45))

Abstract

Protoplasts have been isolated from a handful of forest tree species, and cultured in a few (see 1,2). Because of inadequate knowledge on the in vitro growth and differentiation requirements of cells from forest tree species, it has been extremely difficult to obtain sustained cell division/callus formation from forest tree protoplast cultures. Following isolation, cell wall formation and cell division have been observed in Pinus pinaster and Biota orientalis (3), Picea excelsa (4) and colony/callus formation in Pseudotsuga menziesii (5). So far it has not possible to regenerate whole plants from forest tree protoplasts. We have been able to isolate exceptionally large mesophyll protoplasts, here designated Mega protoplasts from a forest tree species, Populus tremula (aspen). Here we report on the developmental potential of mega as well as normal protoplasts of aspen.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 54.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 69.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Ahuja, M. R. (1981). In Coll. Int. sur la Culture “In Vitro” des Essences Forestieres, AFOCEL, Nangis, France, pp. 315–337.

    Google Scholar 

  2. Ahuja, M. R. (1982). Silvae Genetica 31: 66–77.

    Google Scholar 

  3. David, H., David, A., and Mateile, T. (1981). In Coll. Int. sur la Culture des Essences Forestieres, AFOCEL, Nangis, France, pp. 338–347.

    Google Scholar 

  4. Strmen, J., and Cierna, M. (1981). Ibid pp. 355–358.

    Google Scholar 

  5. Kirby, E. G. (1981). Ibid pp. 348–354.

    Google Scholar 

  6. Lloyd, G., and McCown, B. H. (1981). Proc. Int. Plant Prop. Soc. 30: 421–427.

    Google Scholar 

  7. Wetter, L. C., and Constabel, F. (1982) Plant Tissue Culture Methods, National Research Council of Canada, Saskatoon.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Federal Research Center for Forestry and Forest Products, Institute of Forest Genetics and Forest Tree Breeding, Sieker Landstrasse 2, D-2070, Grosshansdorf, Federal Republic of Germany

    M. R. Ahuja

Authors
  1. M. R. Ahuja
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Basel, Switzerland

    I. Potrykus , A. Hinnen , P. J. King  & R. D. Shillito , ,  & 

  2. ZĂĽrich, Switzerland

    C. T. Harms  & R. Hütter  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1983 Springer Basel AG

About this chapter

Cite this chapter

Ahuja, M.R. (1983). Developmental Potential of Mega and Normal Protoplasts in Populus. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_13

Download citation

  • DOI: https://doi.org/10.1007/978-3-0348-6556-2_13

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6557-9

  • Online ISBN: 978-3-0348-6556-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation