Abstract
Most of the plant protoplasts so far studied were generally isolated from leaf mesophyll tissue. The most notable disadvantage in using leaf tissue is the tedious method of removing epidermis from the leaves which again is not easy in all cases. But the protoplasts can “be easily isolated from any young hypocotyl or epicotyl segments by slicing them in an enzyme solution which also requires minimum in volume. Again the regeneration in legumes, specially in *V. unguiculata is very rare. Thus the new source of protoplast can be tried for regeneration. In having large number of meristematic cells in these hypocotyl segments, it may divide rapidly and might “be an ideal material for regeneration. Lastly, the hypocotyl protoplasts were irradiated with different dose of X-rays to explore radiobiological effects during different phases of cellular development.
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Reference
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© 1983 Springer Basel AG
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Roy, S.C., De, K.K. (1983). Study of Hypocotyl and Mesophyll Protoplasts. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_10
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DOI: https://doi.org/10.1007/978-3-0348-6556-2_10
Publisher Name: Birkhäuser, Basel
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