Summary
Nine proteins have been studied by Polarographic catalytic reaction. It was recommended to define the Polarographic B constant as the current in μA per mm2 of the surface area of the electrode at micromolar concentration of the protein. The examination of uncoiled proteins, denatured by guanidinium chloride and reduced by β-mercaptoethanol, has shown the increase of the catalytic effect of some proteins, especially of serum albumin. The question of “polarographically free and buried groups” is discussed from the “Polarographie catalytic increment” of the half cystine residue point of view.
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© 1971 Springer Basel AG
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Kalous, V. (1971). Polarographic Research of Proteins and Related Substances. In: Milazzo, G., Jones, P.E., Rampazzo, L. (eds) Biological Aspects of Electrochemistry. Experientia Supplementum, vol 18. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5848-9_31
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DOI: https://doi.org/10.1007/978-3-0348-5848-9_31
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-5850-2
Online ISBN: 978-3-0348-5848-9
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