Summary
The Crustacean hyperglycemic hormone (CHH) is one of the best known crustacean neuropeptides, with a potent hyperglycemic action. CHH originates in the neurosecretory perikarya of the eyestalk ganglia. Here we describe the cDNA cloning approach for the complete coding region of a prepro CHH. A cDNA library of X- organ tissue of Carduus maenas was established and screening of this library with an oligonucleotide probe yielded one positive clone. Sequencing data of the clone revealed a precursor structure consisting of a putative 26 amino acid signal peptide, a 38 amino acid peptide of unknown function (peptide C) and the 72 amino acid CHH.
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© 1990 Springer Basel AG
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Gromoll, J., Weidemann, W. (1990). Cloning and Sequencing of the Crustacean Hyperglycemic Hormone from Carcinus Maenas . In: Wiese, K., Krenz, WD., Tautz, J., Reichert, H., Mulloney, B. (eds) Frontiers in Crustacean Neurobiology. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5689-8_44
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DOI: https://doi.org/10.1007/978-3-0348-5689-8_44
Publisher Name: Birkhäuser, Basel
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Online ISBN: 978-3-0348-5689-8
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