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Cloning and Sequencing of the Crustacean Hyperglycemic Hormone from Carcinus Maenas

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Frontiers in Crustacean Neurobiology

Part of the book series: Advances in Life Sciences ((ALS))

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Summary

The Crustacean hyperglycemic hormone (CHH) is one of the best known crustacean neuropeptides, with a potent hyperglycemic action. CHH originates in the neurosecretory perikarya of the eyestalk ganglia. Here we describe the cDNA cloning approach for the complete coding region of a prepro CHH. A cDNA library of X- organ tissue of Carduus maenas was established and screening of this library with an oligonucleotide probe yielded one positive clone. Sequencing data of the clone revealed a precursor structure consisting of a putative 26 amino acid signal peptide, a 38 amino acid peptide of unknown function (peptide C) and the 72 amino acid CHH.

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© 1990 Springer Basel AG

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Gromoll, J., Weidemann, W. (1990). Cloning and Sequencing of the Crustacean Hyperglycemic Hormone from Carcinus Maenas . In: Wiese, K., Krenz, WD., Tautz, J., Reichert, H., Mulloney, B. (eds) Frontiers in Crustacean Neurobiology. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5689-8_44

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  • DOI: https://doi.org/10.1007/978-3-0348-5689-8_44

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-5691-1

  • Online ISBN: 978-3-0348-5689-8

  • eBook Packages: Springer Book Archive

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