Summary
The primary structures and posttranslational modifications of the two major hydrophobic surfactant proteins have been determined by chemical means. They were found to be entirely different for the two molecules and presented special analytical problems. The solubility properties, the presence of extremely hydrophobic polypeptide segments, the existence of truncated forms, and the covalent linkage of fatty acids severely complicated ordinary analyses. This necessitated the use of modified methods.
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Curstedt, T., Jörnvall, H., Robertson, B., Bergman, T., and Berggren, P. (1987) Eur. J. Biochem. 168, 255–262.
Curstedt, T., Johansson, J., Barros-Söderling, J., Robertson, B., Nilson, G., Westberg, M., and Jörnvall, H. (1988) Eur. J. Biochem. 172, 521–525.
Curstedt, T., Johansson, J., Persson, P., Eklund, A., Robertson, B., Löwenadler, B., and Jörnvall, H. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 2985–2989.
Emrie, P. A., Shannon, J. M., Mason, R. J., and Fisher, J. H. (1989) Biochim. Biophys. Acta 994, 215–221.
Fisher, J. H., Shannon, J. M., Hofmann, T., and Mason, R. J. (1989) Biochim. Biophys. Acta 995, 225–230.
Glasser, S. W., Korfhagen, T. R., Weaver, T., Pilot-Matias, T., Fox, J. L., and Whitsett, J. A. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4007–4011.
Glasser, S. W., Korfhagen, T. R., Weaver, T. E., Clark, J. C, Pilot-Matias, T., Meuth, J., Fox, J. L., and Whitsett, J. A. (1988) J. Biol. Chem. 263, 9–12.
Hawgood, S., Benson, B. J., Schilling, J., Damm, D., Clements, J. A., and White, R. T. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 66–70.
Jacobs, K. A., Phelps, D. S., Steinbrink, R., Fisch, J., Kriz, R., Mitsock, L., Dougherty, J. P., Taeusch, H. W., and Floros, J. (1987) J. Biol. Chem. 262, 9808–9811.
Johansson, J., Curstedt, T., Robertson, B., and Jörnvall, H. (1988a) Biochemistry 27, 3544–3547.
Johansson, J., Jörnvall, H., Eklund, A., Christensen, N., Robertson, B., and Curstedt, T. (1988b) FEBS Lett. 232, 61–64.
Johansson, J., Curstedt, T., and Jörnvall, H. (1990) Biochemistry, submitted.
Kostka, S., Kraft, R., Otto, A., and Etzold, G. (1990) J. Prot. Chem. 9, 260–261.
Machleidt, W., and Hofner, H. (1982) In: Methods in Protein Sequence Analysis (M. Elzinga Ed), Humana Press, Clifton, New Jersey, pp 173–180.
Possmayer, F. (1988) Am. Rev. Respir. Dis. 138, 990–998.
Shapiro, D. L., and Notter, R. H. (Eds.) (1989) Surfactant Replacement Therapy. Alan R. Liss, New York
van Golde, L. M. G., Batenburg, J. J., and Robertson, B. (1988) Physiol. Rev. 68, 374–455.
Warr, R. G., Hawgood, S., Buckley, D. I., Crisp, T. M., Schilling, J., Benson, B. J., Clements, J. A., and White, R. T. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 7915–7919.
White, R. T., Damm, D., Miller, J., Spratt, K., Schilling, J., Hawgood, S., Benson, B., and Cordeil, B. (1985) Nature 317, 361–363.
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Johansson, J., Curstedt, T., Persson, P., Robertson, B., Löwenadler, B., Jörnvall, H. (1991). Hydrophobic Surfactant Proteins SP-B and SP-C: Special Analytical Problems. In: Jörnvall, H., Höög, JO., Gustavsson, AM. (eds) Methods in Protein Sequence Analysis. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5678-2_19
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DOI: https://doi.org/10.1007/978-3-0348-5678-2_19
Publisher Name: Birkhäuser, Basel
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