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Using Fluorescence to Study Actomyosin in Yeasts

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Book cover Fluorescent Methods for Molecular Motors

Part of the book series: Experientia Supplementum ((EXS,volume 105))

Abstract

This year marks the 30th anniversary of the first description of the cellular distribution of actin within a yeast cell. Since then advances in both molecular genetics and imaging technologies have ensured research within these simple model organisms has blazed a trail in the field of actomyosin research. Many yeast proteins and their functions are functionally conserved in human cells. This, combined with experimental speed, minimal cost and ease of use make the yeasts extremely attractive model organisms for researching diverse cellular processes, including those involving actomyosin. In this chapter, current state-of-the-art fluorescence methodologies being applied to yeast actomyosin research, together with an honest appraisal of their limitations, such as the pitfalls that should be considered when fluorescently labelling proteins interacting within a dynamic cytoskeleton, will be discussed. Papers describing the established techniques developed for yeast localisation studies will be highlighted. This will provide the reader with an informed overview of the arsenal of imaging techniques available to the yeast actomyosin researcher and encourage them to consider novel ways these simple unicellular eukaryotes could be used to address their own research questions.

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Abbreviations

ABP:

Actin-binding protein

Arp:

Actin-related protein

CAR:

Cytokinetic actomyosin ring

CCD:

Charge couple device

CMOS:

Complementary metal oxide semiconductor

emCCD:

Electron multiplying charge couple device

FP:

Fluorescent protein

Tm:

Tropomyosin

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Acknowledgements

Dan Mulvihill is a Royal Society Industry Fellow. Research within his lab is funded by the Royal Society and the BBSRC.

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  1. School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ, UK

    Daniel P. Mulvihill

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  1. Daniel P. Mulvihill
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Correspondence to Daniel P. Mulvihill .

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  1. Chromosome Organisation and Dynamics, Max-Planck Institute of Biochemistry, Martinsried, Germany

    Christopher P. Toseland

  2. Department of Cellular Physiology, Ludwig-Maximilians-Universität München, Munich, Germany

    Natalia Fili

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Mulvihill, D.P. (2014). Using Fluorescence to Study Actomyosin in Yeasts. In: Toseland, C., Fili, N. (eds) Fluorescent Methods for Molecular Motors. Experientia Supplementum, vol 105. Springer, Basel. https://doi.org/10.1007/978-3-0348-0856-9_13

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