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Twenty Years of G-CSF pp 25–40Cite as

Mouse Models of G-CSF Signaling in Hematopoiesis

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Part of the book series: Milestones in Drug Therapy ((MDT))

Abstract

To begin to define the role of granulocyte colony-stimulating factor (G-CSF) in the regulation of hematopoiesis, Lieschke and colleagues generated G-CSF−/− (knockout) mice [1]. Knockout mice are genetically engineered to contain a complete loss-of-function (null) mutation of the gene of interest. They are generated by homologous recombination in murine embryonic stem cells in which the normal gene (in this case Csf3 encoding G-CSF) is replaced with a mutated gene. The targeted embryonic stem cells are implanted into pseudopregnant mice to generate a transgenic mouse line carrying the mutated gene. Importantly, the rest of the murine genome is intact, allowing investigators to examine the effect of the loss of that gene, in isolation, on a biologic process.

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Acknowledgments

DCL is supported by grants from the NIH (RO1 CA136617; RC2 CA1455073; and RO1 HL60772) and by the Leukemia & Lymphoma Society TRA 6030-10). The author thanks Mahil Rao for the generation of Fig. 3.

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  1. Division of Oncology, Washington University, 660 S. Euclid Avenue, 8007, St. Louis, MO, 63110, USA

    Daniel C Link

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Correspondence to Daniel C Link .

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  1. Amgen Inc., One Amgen Center Drive, Thousand Oaks, 91320, California, USA

    Graham Molineux

  2. MA Foote Associates, Par Five Court 4284, Westlake Village, 91362, California, USA

    MaryAnn Foote

  3. Amgen, Inc., One Amgen Center Dr. 1, Thousand Oaks, 91320, California, USA

    Tara Arvedson

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Link, D.C. (2012). Mouse Models of G-CSF Signaling in Hematopoiesis. In: Molineux, G., Foote, M., Arvedson, T. (eds) Twenty Years of G-CSF. Milestones in Drug Therapy. Springer, Basel. https://doi.org/10.1007/978-3-0348-0218-5_3

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