Abstract
Indiscriminate disposal of solid wastes into the environment generates landfill gases and leachates. Leachate, a mixture of deleterious substances including metals, inorganic and organic compounds, particulate matters, radioactive elements, and microorganisms, contaminates soils and underground and surface water resources increasing risk to human and wildlife health. This study is a compendium of studies on the cytotoxic and genotoxic assessment of Olusosun landfill (the largest unregulated landfill in African). Leachates emitted from Olusosun solid waste landfill induced cytotoxicity and DNA damage via direct and indirect mechanisms. Different biomarkers of genotoxicity were used to evaluate the cytotoxic and genotoxic potentials of OSL leachates. Biomarkers of chromosome aberration, micronucleus assay, comet assay, sperm head abnormality test and toxicogenomic signatures, were assessed in somatic and germline cells of exposed organisms. The mechanisms of DNA damage include generation of free radicals and oxidative stress, inhibition of DNA repair process, alkylation on DNA molecules that may lead to chromosome breakage, down-regulation of transcriptional genes related to apoptotic expressions, and increased DNA fragmentation in cells and tissue. The damaged DNA can increase genome instability and genetic related diseases. Heavy metals, physico-chemicals and organic substances present in the leachates are known mutagens, genotoxin and carcinogens. It is suggested that proper landfill management be instituted in various countries to avert exposure to landfill emissions.
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References
Adedara IA, Lawal TA, Adesina AA, Oyebiyi OO, Ebokaiwe AP, Farombi EO (2014) Sperm functional parameters and erythrocytes oxidant–antioxidant imbalance during municipal landfill leachate treatment withdrawal in rats. Environ Toxicol Pharmacol 37:460–467
Adedara IA, Oyebiyi OO, Lawal TA, Adesina AA, Farombi EO (2013) Involvement of oxidative stress in municipal landfill leachate-induced toxicity in boar sperm. Environ Toxicol Pharmacol 36:972–978
Ademola OJ, Alimba CG, Bakare AA (2020) Reproductive toxicity assessment of Olusosun municipal landfill leachate in Mus musculus using abnormal sperm morphology and dominant lethal mutation assays. Environ Anal Health Toxicol 35(2) (Article ID: e2020010, 10 pages). https://doi.org/10.5620/eaht.e2020010
Alimba CG, Adewumi OO, Binuyo OM, Odeigah PGC (2021) Wild black rats (Rattus rattus Linnaeus, 1758) as zoomonitor of genotoxicity and systemic toxicity induced by hazardous emissions from Abule Egba unsanitary landfill, Lagos, Nigeria. Environ Sci Pollut Res Int 28(9):10603–10621
Alimba CG, Bakare AA (2016) In vivo micronucleus test in the assessment of cytogenotoxicity of landfill leachates in three animal models from various ecological habitats. Ecotoxicology 25:310–319
Alimba CG, Bakare AA, Aina OO (2012) Liver and kidney dysfunction in Wistar rats exposed to municipal landfill leachate. Resour Environ 2(4):150–163
Alimba CG, Bakare AA, Latunji CA (2006) Municipal landfill leachates induced chromosome aberration in rat bone marrow cells. Afr J Biotechnol 5(22):2053–2057
Alimba CG, Gandhi D, Sivanesan S, Bhanarkar MD, Naoghare PK, Bakare AA, Krishnamurthi K (2016) Chemical characterization of simulated landfill soil leachates from Nigeria and India and their cytotoxicity and DNA damage inductions on three human cell lines. Chemosphere 164:469–479
Alimba CG, Onajole AI, Bakare AA (2015) Brain dysfunctions in Wistar rats exposed to municipal landfill leachates. Beni-Suef Univ J Basic Appl Sci 4:284–290
Alimba CG, Rudrashetti AP, Sivanesan S, Krishnamurthi K (2022) Landfill soil leachates from Nigeria and India induced DNA damage and alterations in genes associated with apoptosis in Jurkat cell. Environ Sci Pollut Res 29(4):5256–5268
Alimba CG, Sivanesan S, Krishnamurthi K (2022) Mitochondrial dysfunctions elicited by solid waste leachates provide insights into mechanisms of leachates induced cell death and pathophysiological disorders. Chemosphere 307:136085. https://doi.org/10.1016/j.chemosphere.2022.136085
Alimba CG (2013) DNA and systemic damage induced by landfill leachates and health impacts of human exposure to landfills in Lagos and Ibadan, Nigeria. Ph.D thesis. Department of Zoology, University of Ibadan, Nigeria, pp 1–258
Alter BP, Rosenberg PS, Giri N, Baerlocher GM, Lansdorp PM, Savage SA (2012) Telomere length is associated with disease severity and declines with age in dyskeratosis congenita. Haematologica 97(3):353–359
Annepu RK (2012) Sustainable Solid Waste Management in India. MSc thesis. Department of Earth and Environmental Engineering, Fu Foundation School of Engineering and Applied Science, Columbia University in the City of New York, pp 1–189
Aralu CC, Okoye PC, Akpomie KG, Chukwuemeka-Okorie HO, Abugu HO (2022) Polycyclic aromatic hydrocarbons in soil situated around solid waste dumpsite in Awka, Nigeria. Toxin Rev 42(1):122–131. https://doi.org/10.1080/15569543.2021.2022700
Boyle WC, Ham RK (1974) Biological treatability of landfill leachate. J Water Pollut Control Feder 46:860–872
Christensen TH, Kjeldsen P, Bjerg PL, Jensen DL, Christensen JB, Baun A, Albrechtsen HJ, Heron C (2001) Biogeochemistry of landfill leachate plumes. Appl Geochem 16:659–718
Dhawan A, Bajpayee M, Parmar D (2009) Comet assay: a reliable tool for the assessment of DNA damage in different models. Cell Biol Toxicol 25:5–32
de Diana DF, Abreu JS, Serafini DC, Ortız JF, Samaniego MJ, Aranda AC, Zamorano-Ponce E (2018) Increased genetic damage found in waste picker women in a landfill in Paraguay measured by comet assay and the micronucleus test. Mutat Res 836:19–23
El-Fadel M, Findikakis AN, Leckie JO (1997) Environmental impacts of solid waste landfilling. J Environ Manag 50:1–25
De Felice B, Nappi C, Zizolfi B, Guida M, Di Spiezio Sardo A, Bifulco G, Guida M (2012) Telomere shortening in women resident close to waste landfill sites. Gene 500:101–106
Freeman A (2012) Seven of the largest landfills in the world. Takepart.com https://en.wikipedia.org/wiki/Olusosun_landfill. Accessed 2 Jan 2022
Gajski G, Orescanin V, Vera G-V (2012) Chemical composition and genotoxicity assessment of sanitary landfill leachate from Rovinj, Croatia. Ecotoxicol Environ Saf 78:253–259
Gardiner R, Hajek P (2020) Municipal waste generation, R&D intensity, and economic growth nexus—a case of EU regions. Waste Manage 114:124–135
Gates KS (2009) An overview of chemical processes that damage cellular DNA: spontaneous hydrolysis, alkylation, and reactions with radicals. Chem Res Toxicol 22(11):1747–1760
Gbadebo AM, Alabi OA, Alimba CG, Bakare AA (2022) Metal bioaccumulation, cytogenetic and clinico‑biochemical alterations in Rattus norvegicus exposed in situ to a municipal solid waste Landfll in Lagos, Nigeria. Biol Trace Element Res. https://doi.org/10.1007/s12011-021-02758-3
Gilbreath S, Kass PH (2006) Fetal and neonatal deaths and congenital anomalies associated with open dumpsites in Alaska Native villages. Int J Circumpolar Health 65(2):133–147
Gupta P, Bhargava A, Kumari R, Lodhi L, Tiwari R, Gupta PK, Bunkar N, Samarth R, Mishra PK (2019) Impairment of mitochondrial nuclear cross talk in lymphocytes exposed to landfill leachate. Environ Health Insights 13:1–11
Hartwig A, Asmuss M, Ehleben I, Herzer U, Kostelac D, Pelzer A, Schwerdtle T, Bürkle A (2002) Interference by toxic metal ions with DNA repair processes and cell cycle control: molecular mechanisms. Environ Health Perspect 110(suppl 5):797–799
Jarup L, Morris S, Richardson S, Briggs D, Cobley N, de Hoogh C et al (2007) Down syndrome in births near landfill sites. Prenat Diag 27(12):1191–1196
Jenkins EC, Tassone F, Ye L, Gu H, Xi M, Velinov M, Brown WT, Hagermen RJ, Hagerman PJ (2008) Reduced telomere length in older men with premutation alleles of the fragile X mental retardation 1 gene. Am J Med Genet A 146A(12):1543–1546
Lea DE, Catcheside DG (1942) The mechanism of the induction by radiation of chromosome aberrations in Tradescantia. J Genet 44:216–245
Mills C (2018) 15 of the world’s largest landfills with photos and statistics. Updated on June 6, 2018 (cited 2019 Aug 12). https://owlcation.com/stem/15-of-the-Worlds-Largest-Landfills
Mohammed AA, Iniaghe PO, Abu TO, Bello MO, Abdulkadir MD (2020) Source analysis of heavy metals and polycyclic aromatic hydrocarbons from a popular dumpsite, Lagos State, Nigeria. J Turk Chem Soc 7(2):489–504. https://doi.org/10.18596/jotcsa.687322
Morales ME, Derbes RS, Ade CM, Ortego JC, Stark J, Deininger PL, Roy-Engel AM (2016) Heavy metal exposure influences double strand break DNA repair outcomes. PLoS ONE 11(3):e0151367. https://doi.org/10.1371/journal.pone.0151367
National Environmental Standards and Regulation Enforcement Agency (Federal Republic of Nigeria Official Gazette) (2011) National Environmental (sanitation and waste control). Published by Federal Government of Nigeria printer, Abuja FGP 112/102009/L000 (OL54) 60(96):1057–1102
Ncube F, Ncube EJ, Voyi K (2017) A systematic critical review of epidemiological studies on public health concerns of municipal solid waste handling. Perspect Public Health 137(2):102–108
Ogundiran OO, Afolabi TA (2008) Assessment of the physico-chemical parameters and heavy metals’ toxicity of leachates from municipal solid waste open dumpsite. Int J Environ Sci Technol 5(2):243–250
Ogungbemi KI, Adedokun MB, Ibitoye AZ, Oyebola OO, Owoade RL (2023) Estimation of radiological impact of the activities of Olusosun Dump Site on workers and dwellers of Olusosun, in Lagos Southwest Nigeria. J Radiat Res 64(1):53–62. https://doi.org/10.1093/jrr/rrac067
Ogunyemi AK, Samuel TA, Amund OO, Ilori MO (2018) Genotoxicity evaluation of landfill solid waste leachates from a waste dumpsite in Lagos State, Nigeria using the Allium cepa Assay. Plant Animal Res J 1(3):68–76
Ogwueleka TC (2009) Municipal solid waste characteristics and management in Nigeria. Iran J Environ Health Sci Eng 6:173–180
Ostling D, Johanson KJ (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123(1):291–298
Oziegbe O, Oluduro AO, Oziegbe EJ, Ahuekwe EF, Olorunsola SJ (2021) Assessment of heavy metal bioremediation potential of bacterial isolates from landfill soils. Saudi J Biol Sci 28:3948–3956
Pimienta-Serrano EV, Pacheco-Bustos C (2022) Perspectives on the environmental impact of anthropogenic activities and the generation of solid waste in beaches of the Colombian Caribbean. Environ Eng 24(2):1–12. https://doi.org/10.25100/iyc.v24i2.11365
Sang N, Li G (2004) Genotoxicity of municipal landfill leachate on root tips of Vicia faba. Mutat Res 560:159–165
Sang N, Li G (2005) Chromosomal aberrations induced in mouse bone marrow cells by municipal landfill leachate. Environ Toxicol Pharmacol 20:219–224
Shekha YA, Al-Attar MS, Saleem MA, Toma JJ, Abdulla SM (2017) Effect of landfill leachates extract of Erbil city on abnormal sperm morphology and chromosomal aberrations in male albino mice. ZANCO J Pure Appl Sci 29(6):18–27
Singh NP, Mccoy MT, Tice RR, Schneider EI (1988) A simple technique for quantitation of low levels of DNA damage in Individual cells. Exp Cell Res 175:184–191
Styrna J, Kilarski W, Krzanowska H (2003) Influence of the CBA genetic background on sperm morphology and fertilization efficiency in mice with a partial Y chromosome deletion. Reproduction 126:579–588
Toure A, Szot M, Mahadevaiah SK, Rattigan A, Ojarikre OA, Burgoyne PS (2004) A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166:901–912
USEPA (1998) U.S. Environmental protection agency. Characterization of municipal solid waste in the United States: 1997 Update. EPA/530-R-98-007
United Nation (2013) Population Division, Department of Economic and Social Affairs of the United Nations Secretariat. World Population Prospects: the 2012 Revision (New York)
United States Environmental Protection Agency. National recommended water quality criteria 2006
United States Environmental Protection Agency (USEPA) (1997) Greenhouse gas emissions from the management of some selected materials in municipal solid wastes. Document prepared for Office of Solid Waste and Office of Policy, Planning and Evaluation, U.S Environmental Protection Agency by ICF incorporated EPA Contract No. 68-W6-0029 Work Assignment 0-06. https://nepis.epa.gov/Exe/ZyNET.exe/1000118X.TXT?
Vrijheid M, Dolk H, Armstrong B, Boschi G, Busby A, Jorgensen T, Pointer P (2002) Hazard potential ranking of hazardous waste landfill sites and risk of congenital anomalies. Occup Environ Med 59(11):768–776
Wyrobek AJ, Bruce WR (1975) Chemical induction of sperm abnormalities in mice (25 chemicals/mutagens/teratogens/carcinogens). Proc Natl Acad Sci USA 72:4425–4429
Wyrobek AJ, Gordon LA, Burkhart JG, Francis MW, Kapp RW Jr, Letz G, Malling HV, Topham JC, Whorton MD (1983) An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals: a report of the U.S. environmental protection agency Gene-Tox program. Mutation Res 115(1):1–72
Xu G, Zhao J, Yu H, Wang C, Huang Y, Zhao Q, Zhou X, Li C, Liu M (2022) Structural insights into the mechanism of high-affinity binding of ochratoxin A by a DNA aptamer. J Am Chem Soc 144(17):7731–7740
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Alimba, C.G. (2024). Biological Test Used in the Assessment of Cytotoxicity and Genotoxicity of Olusosun Landfill: The Largest and Unregulated Landfill in Nigeria. In: Anouzla, A., Souabi, S. (eds) A Review of Landfill Leachate. Springer Water. Springer, Cham. https://doi.org/10.1007/978-3-031-55513-8_2
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