Abstract
In Morocco, the sour orange (Citrus aurantium L.) is the most anciently used rootstock, it remains dominant. Due to its better affinity with most commercial varieties, its adaptation to a wide range of soils and mainly due to its resistance to root rot caused by Phytophthora spp. However, it gives susceptible associations to tristeza. In vivo, several methods were used for the screening of citrus rootstocks in relation to Phytophthora spp. The objective of our experimentation is the development of an in vitro screening test of rootstocks caused by Phytophthora parasitica. Plants of Citrumelo (57. 98. 502), Poncirus Trifoliata, Citron troyer, Citron Troyer c 35 B 6 A11, Cleopatra mandarin tree x CC 30 575, Cleopatra mandarin tree xP.T 30 584, Cleopatra mandarin tree x cc B2 30 576, Mandarin tree changsa B2, Mandariniecleop X CC 30,577 and a South African Hybrid aged 2 months were watered daily and fertilized every 15 days using a nutrient solution. Inoculation was done by soaking the root systems in a spore suspension of P. parasitica for 24 h. Inoculation with P. parasitica caused root rot (Rot-tips) with a reduction in feeder fiber production and colonization of root systems that varied between rootstocks. Indeed, the Mandarin hybrids Cleopatra x cc 30 575 and the Mandariner Cleopatra x cc B2 30 576, were the most susceptible rootstocks to P. parasitica while the hybrids Citrange Troyes, Citrange Troyer C 35 B 6 A11 and the hybrid of South Africa are the most resistant rootstocks. Variability of rootstocks colonization by P. parasitica is thought to be variability in the production ability of 6, 7-dimethoxycoumarin (DMC) in root tissues of rootstocks.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Afek U, Sztejnberg A (1990) A rapid method for evaluating citrus seedlings for resistance to foot rot caused by Phytophthora citrophthora. Plant Dis 74:66–68
Affailal A (1987) Manifestation de la verticilliose sur les tomates sensibles et résistantes : effet de la salinité sur le développement des deux races de Verticillium dahliae Kleb. et sur la réaction des plantes à l'agent pathogène. Fac Sci Rabat thèse, Maroc, 127p
Agostini JP, Timmer LW, Castle WS, Mitchell DJ (1991) Effect of citrus rootstocks on soil populations of Phytophthora parasitica. Plant Dis 75:296–300
Bénaouf G (2005) (CIVAM BIO CORSE) Corse Pôle agronomique—20230 San SAN GIULIANO. Produire des agrumes en agriculture biologique
Benyahia H (1993) Evaluation de la résistance de 18 clones du bigaradier à la pourriture racinaire due à Phytophthora citrophthora et Phytophthora parasitica. Mémoire présentée à la faculté des Sciences Semlalia pour l'obtention du certificat des études approfondies en physiologie végétale, 70p
Benyahia H (1998) Effet de la salinité sur le développement des maladies à Phytophthora des agrumes au Maroc. Thèse de 3ème cycle, Université Cadi Ayyad, Faculté des Sciences Semlalia, Marrakech, 170 pp
Benyahia H (2007) Amélioration de la résistance des porte-greffes d’agrumes vis-à-vis des contraintes biotiques et abiotiques, thèse de Doctorat. Université Mohamed Ben Abdellah, Faculté des Sciences Dhar El Mahraz
Benyahia H, Ait HM, M., Jrifi A. and Lamsettef Y. (2004) Effet de la salinité de l’eau d’irrigation sur la colonisation des racines des porte-greffes d’agrumes par Phytophthora parasitica. Fruits 59(2004):101–108
Benyahia H, Beniken L, Omari FE, Benazzouze A, Handaji N, Msatef Y, Olitrault P (2011) Evaluation of the resistance of few citrus rootstocks to alkalinity by applying a fast test of screening. Afr J Agric Res 6(4):780–784
Boava LP, Cristofani-Yaly M, Stuart RM, Machado MA (2011) Expression of defenserelated genes in response to mechanical wounding and Phytophthora parasitica infection in Poncirus trifoliata and Citrus sunki. Physiol Mol Plant Pathol 77:1–7
Boudoudou D, Fadli A, Talha A, Bourachdi Y, Douira A, Benyahia H (2015) Effect of seasonal and citrus rootstocks on inoculum density of Phytophthora sp. inCitrus orchard in a heavy soil of the Gharb region of Morocco. Biolife 3(2):367–377. https://doi.org/10.17812/blj2015.32.4
Carpenter JW, Furr JR (1962) Evaluation of tolerance to root rot caused by Phytophthora parasitica in seedlings of citrus and related genera. Phytopathology 52:1277–1285
Dalal B, Abdelhak T, Anas F, Allal D, Hamid B (2016) Influence of citrus rootstocks on soil populations of phytophthora sp. In the gharb region in morocco. Int J Recent Sci Res 7(11):14230–14236
Graham JH (1990) Evaluation of tolerance of citrus roostocks to phytophthora root rots in chlamydospore-infested soil. Am Phytopathol Soc 74(10):743–746
Graham JH (1995) Root regeneration and tolerance of citrus roots tocks to root rot caused by Phytophtora nicatianae. Phytopatholgy 85:111–117
Graham J, Feichtenberger E (2015) Citrus Phytophthora diseases: management challenges and successes. J Citrus Pathol 2(1):1–11
Graham JH, Timmer LW, Drouillard DL, Peever TL (1998) Characterization of Ph tophthora spp. causing outbreaks of citrus brown rot in Florida. Phytopathology 88:724–729
Graham JH, Timmer LW, Dewdney MM (2019) Florida citrus pest management guide: phytophthora foot rot and root rot. The Plant Pathology Department, UF/IFAS Extension, IFAS publication, Florida, pp 156. 7 p
Jim G, Irey M, Taylor J (2011) Phytophthora damage to roots A potential contributor to decline of HLB affected Trees. Citrus Ind
Najwa B, Metoui N, Naïma Boughalleb-M’Hamdi. (2016) Screening fortolerance of different citrus rootstocks against zoospores of Phytophthora nicotianae in infested soil. J Phytopathol Pest Manage 3(3):63–75
Ouissam K (2014) Erosion génétiquedes espèces agrumicolesdans la wilaya de Skikda: Contraintes de production, Université 20 Août 1955 Skikda
Serrhini MN (1986) La résistance de Phytophthora citrophthora au metalaxyl. Université catholique de Louvain Belgique, Thèse de Doctorat, p 150
Sulistyowati L (1993) Effect of salinity on development of root infections caused by Phytophthora citrophthora in Citrus rootstocks growing in hydroponics. Agrivita 16(1):13–19
Sulistyowati L, andKeane, P. J. (1992) Effect of soil salinity and water content on stem rot caused by Phytophthora citrophthora and accumulation of phytoalexines in Citrus rootstocks. Phytopathology 82:771–777
Sulistyowati L, Keane PJ, Anderson JW (1990) Accumulation of the phytoalexin,6,7-dimethoxycoumarin, in roots and stems of Citrus seedlings following inoculation with Phytophthoracitrophthora. Physiol Mol Plant Pathol 37:451–461
Timmer, LW„ Garnsey, SM., Graham, JH. (2000) Compendium of citrus diseases, 2nd edn. APS Press Inc., St. Paul, MN, USA
Vanderweyen A (1974) La gommose à Phytophthora des agrumes au Maroc. Les agents pathogènes et la recherche de porte-greffe résistants. Al-Awamia 51:83–127
Whiteside JO, Garnsey SM, Timmer LW, eds, (1988). Compendium of Citrus Diseases. St Paul, MN, USA: APS Pres
Zitko SE, Timmer LW, Sandler HA (1991) Isolation of Phy-tophthora palmivora pathogenic to citrus in Florida. Plant Dis 75:532–535
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2024 The Author(s), under exclusive license to Springer Nature Switzerland AG
About this chapter
Cite this chapter
Boudoudou, D., Douira, A., Benyahia, H. (2024). Evaluation of the Resistance of 10 New Citrus Rootstocks to Root Rot Caused by Phytophthora Parasitica. In: Azrour, M., Mabrouki, J., Guezzaz, A. (eds) Sustainable and Green Technologies for Water and Environmental Management. World Sustainability Series. Springer, Cham. https://doi.org/10.1007/978-3-031-52419-6_18
Download citation
DOI: https://doi.org/10.1007/978-3-031-52419-6_18
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-031-52418-9
Online ISBN: 978-3-031-52419-6
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)