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Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material

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Physics and Mechanics of New Materials and Their Applications (PHENMA 2023)

Part of the book series: Springer Proceedings in Materials ((SPM,volume 41))

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Abstract

This study focuses on the advancement of new techniques for cryopreservation of reproductive cells at low temperatures, including sturgeon fish. The approach is based on smart freezing process control. To ensure cryoprotectant and reproductive cells are not damaged prior to vitrification, a piezoactuator is used to stir the mixture. This technique facilitates the intensive mixing of cryoprotectant, promoting the diffusion of the substance through the cell membrane. During direct vitrification, researchers observe a phase transition phenomenon in the presence of crystal formation. This study constructs a mathematical model to investigate this process while comparing numerical experiments to in situ experiments. The results obtained have implications for selecting optimal parameters required for the low-temperature preservation of fish reproductive cells.

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Acknowledgements

This research was supported by the Russian Science Foundation (RSF), grant No. 21-16-00118.

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Authors and Affiliations

  1. Don State Technical University, 1, Gagarin Square, Rostov-on-Don, 344010, Russia

    Andrey Matrosov, Irina Serebryanaya & Daria Nizhnik

  2. Fevzi Yakubov Crimean Engineering and Pedagogical University, Simferopol, Russia

    Arkady Soloviev

  3. Southern Federal University, 8a, Milchakova St, Rostov-on-Don, 344090, Russia

    Olga Pustovalova

Authors
  1. Andrey Matrosov
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  2. Arkady Soloviev
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  3. Irina Serebryanaya
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  4. Olga Pustovalova
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  5. Daria Nizhnik
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Corresponding author

Correspondence to Andrey Matrosov .

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Editors and Affiliations

  1. I. I. Vorovich Institute of Mathematics, Mechanics and Computer Science, Southern Federal University, Rostov-on-Don, Russia

    Ivan A. Parinov

  2. Department of Microelectronic Engineering, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan

    Shun-Hsyung Chang

  3. Institute for Research and Community Service, Universitas 17 Agustus 1945 Surabaya, Surabaya, Indonesia

    Erni Puspanantasari Putri

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Matrosov, A., Soloviev, A., Serebryanaya, I., Pustovalova, O., Nizhnik, D. (2024). Modelling of Phase Transitions in the Process of Cryopreservation of Biological Material. In: Parinov, I.A., Chang, SH., Putri, E.P. (eds) Physics and Mechanics of New Materials and Their Applications. PHENMA 2023. Springer Proceedings in Materials, vol 41. Springer, Cham. https://doi.org/10.1007/978-3-031-52239-0_12

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