Abstract
In the past few decades, advancements have been made in the development of biosensors for the detection and analysis of Deoxyribonucleic Acid (DNA) and genes. The objective of this study was to explore Electrical Bioimpedance Spectroscopy (EBiS) for DNA concentration. EBiS patterns in the complex plane for different concentrations of salmon sperm DNA were obtained, and analyzed to identify potential sensitivity parameters for characterization and calibration curves design. According to the results, characteristic EBiS patterns are associated to the analyte vehicle and its parameters change as a function of the present DNA concentration. It seems possible to determine DNA concentration from measuring reactance (X) and resistance (R) at frequencies centered around 200 and 5 kHz, respectively. Exploration of X and R parameters at such frequencies seems pertinent to design calibration curves to estimate DNA concentration of 73 to 1200 ng/µL in a sample. Further studies are warranted to confirm the observations.
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Acknowledgments
NNGG, CMS and LBM thank to “Programa Delfín” their support to developed this research during Summer Scientific 2023. The Instituto Politécnico Nacional-México supported this work under project SIP-20230467.
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Gómez-González, N.N. et al. (2024). Multifrequency Bioimpedance Characterization of DNA Concentration: Sensitivity Analysis. In: Flores Cuautle, J.d.J.A., et al. XLVI Mexican Conference on Biomedical Engineering. CNIB 2023. IFMBE Proceedings, vol 96. Springer, Cham. https://doi.org/10.1007/978-3-031-46933-6_40
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DOI: https://doi.org/10.1007/978-3-031-46933-6_40
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